1. High Performance Liquid Chromatography Allyson Lundberg University of Utah November 29, 2006

2. Overview Introduction Theory Results Conclusions/Recommendation Questions

3. Applications Identification Separations Purification

4. Introduction Sugar analysis of Fructose and Sucrose

5. Theory Normal Phase Reversed Phase Size Exclusion Ion Exchange

6. Theory Column Selection –The Luna 5 micrometer Amino Column

7. Theory Sample Chromatograph

8. Theory Sucrose Calibration

9. Theory Fructose Calibration

10. Results Unknown Chromatograph

11. Results Sucrose Concentration 55 +/- 19 g/L (with 95% confidence) Fructose Concentration 186 +/- 22 g/L (with 95% confidence)

12. Conclusions and Recommendations Take precautions to make more precise calibration standards Perform more calibration runs with more standard solutions Perform more unknown runs

13. Summary Many applications for HPLC HPLC Theory Experimental Results Conclusions and Recommendations

14. References The Essential Guide to Analytical Chemistry. Schwedt, Georg. Wiley and Sons, Hoboken, NJ 1997. Analysis of Data. Haile, J. M. Macatea Productions, Central SC, 2003. CRC Handbook of Chemistry and Physics, 49 th College Edition. Edited by Weast, Robert C. The Chemical Rubber Company, Cleveland, OH, 1968 “High Performance Liquid Chromatography,” Unknown author, http://en.wikipedia.org/, November 23, 2006 http://en.wikipedia.org/

15. Questions?