1. Drosophila Identification of various phenotypes.
Biology 22
Drosophila
Identification of various phenotypes.

2. Outline Brief description of Drosophila phenotypes and life cycle
1-Experiment 1: collection of data related to gender and body color
2-Experiment : 3 point Test Cross
How to study keep your flies still during the identification.

3. Reference for Pictures:
Carolina Drosophila melanogster Manual ( )
Published: Carolina Biological Supply Company
Based on research of Dr. Thomas Hunt Morgan

5. The life Cycle

6. Life Cycle: Drosophila melanogaster
Eggs: contain filaments
2 molting phases “instars”
Life cycle is 10 days

7. Fruity Fly Egg and Larva

8. Fruit Fly Pupa and Adult

9. 1- EXPERIMENT: Identification of:
Gender
Body Color

10. Wild Type Versus Mutants
Body Color
Wild type: Beige
Mutant: Yellow body

11. Parental phenotypes Experiment: body color
A= female: wt (beige)
B=female: wt (beige)
C=male: wt (beige)
D=female: mutant (yellow)
E=male: mutant (yellow)

12. Fruit Fly Body Color

13. Sex combs: Male Drosophila

14. Female and Male Fruit Flies

15. Abdominal segements 7 segments to female abdominal segments
5 segments to male abdominal segments
Male body size is smaller than female
But the “last” abdominal segment is larger in male

17. 2- Experiment: 3 point test cross:
Eye color
Body color
Wing length

18. 3 point test cross for: 1. Body Color 2. Wing length 3. Eye Color
♦ Wt vs Mutant
Beige (bl+) vs. Black (bl)
Long(vg+) vs. Vestigal(vg)
Brick red (br+) vs. brown (br)

19. Crosses:
Female Males
Males and female progeny from this F2 cross are your 3 point test cross results!
bl+ vg+ Bw+
bl vg bw X
bl vg bw
bl vg bw

20. There are all sorts of eyes!

21. Eyes

22. You will have only red eyes (wt)

23. Fruit Fly Wings

24. Nap Time Triethylamine

25. How to anesthetize a fly

26. How to use the Napper

27. View the Fruit Fly

28. You will record class data
After counting the crosses record the data on the class spreadsheet