1. DNA Isolation

2. Nucleic Acid Structure & Function DNA & RNA are composed of Nucleotides A nucleotide consists of three covalently-linked parts: –A nitrogen containing base (Pyrimidines & Purines –A five carbon sugar (deoxyribose and ribose sugars) –One or more phosphate groups

3. Pyrimidines & Purines

4. Hydrogen Bonding

5. DNA Replication is Semi-Conservative Each strand of DNA is used as a template for it’s partner Therefore each daughter DNA helix is composed of one parent and one daughter strand

6. DNA Replication is Unidirectional

7. From DNA to Protein Transcription Translation

8. Denaturation & Digestion Denaturation Annealing Digestion via DNAse1

9. Gene Function & Cloning In Bacteria Plasmids - pUC18 Restriction Enzymes Restriction Sites Antibiotic Resistance Transformation Selecting Recombinant Plasmids

10. Electrophoresis Separates DNA fragments based on size

11. PCR Used to “amplify” DNA sequences Primers Polymerase Cycle