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All other DNA profiling steps occur.. PCR, Restriction enzymes, gel electrophoresis or interpretation by current and charge and mass But there are new.

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Presentation on theme: "All other DNA profiling steps occur.. PCR, Restriction enzymes, gel electrophoresis or interpretation by current and charge and mass But there are new."— Presentation transcript:

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3 All other DNA profiling steps occur.. PCR, Restriction enzymes, gel electrophoresis or interpretation by current and charge and mass But there are new procedures….

4 RFLP older procedure longer to process requires large sample

5 DNA polymorphism is found in non-encoded junk DNA… highly variable in length and base sequence

6 Some bases are constantly repeated along lengths of the DNA strand a variable number of times… called VNTR variable number tandem repeats

7 Short tandem repeats: STRs shorter than VNTRs and repeat frequently More known STRs than VNTRs; more data, more validity, less error Even less sample amounts and degraded amounts are useful and quick! Introduced in 1994

8 NOW: PCR and STR is standard and Daubert accepted RFLP: 1/1 billion likelihood of same DNA STR: 1/1 trillion Reliable, fast, works well with very little sample and /or degraded and more precision than RFLP

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