1. Gel Electrophoresis UNIT 5 – DNA THIS IS ON THE WEBSITE SO YOU CAN USE IT FOR YOUR REPORT. Use this PowerPoint to complete writing you lab report. Lab Packet with Data Lab Report Rough draft Lab Report (typed) ALL DUE: Thursday March 5 th, 2015

2. Directions You are writing a lab report on Gel Electrophoresis LAB THIS MUST BE TYPED AT HOME!!! It must look similar to the Rough Draft copy. Please see me if there is a problem. Today you are working on rough draft together. Use this PowerPoint Use your lab paper Use the procedure paper Use your old Yeast Lab as a guide. You are completing all sections!! Title page, abstract, introduction, materials, methods are on the packet. Use a blank sheet of paper to complete observations & data, analysis, and conclusion.

3. People that missed the lab for PARCC or were absent: Read the information on the next slide that has to do with DNA Fingerprinting, this goes along with your PowerPoint….I would take notes on the side and also get notes from your classmates. Read the lab packet and the procedure paper. For this lab, we performed gel electrophoresis. We used dyes instead of real DNA. Basically you have known dyes and unknown dyes. Lanes 1-5 were the known dyes. Lanes 6-8 are unknown MIXTURES (more than one dye). You have to determine based of the known dyes, what is present in the mixtures. This technique of putting the dye (DNA) in the gel is exactly how DNA Fingerprinting is done in labs to determine paternity tests or to solve crimes, compare DNA, etc.

4. DNA Fingerprinting DNA fingerprint is the analysis of DNA fragments to determine whether they come from a particular individual. #1: Obtain the specimen, #2: make copies of it (called PCR – polymerase chain reaction) so that you have a large sample of DNA FRAGMENTS, #3: the fragments are then place in a gel electrophoresis chamber where they can be compared with each other. The fragments travel through the gel leaving bands or marks. If two DNA samples are identical they will have identical marks.

5. BIO 1A Data Lane 1: Purple 1.5 cm Lane 2: Yellow 1 cm Lane 3: Red2.1 cm Lane 4: Blue 0.8 cm Lane 5: Pinknegative movement (can’t measure) Lane 6: Blue, Red 0.9 cm, 2.1 cm Lane 7: Yellow, Purple, Red 0.8 cm, 1.4 cm, 1.8 cm Lane 8: Pink, Blue negative, 0.6 cm

6. BIO 3A Use the following slides to look at your data for each individual group.