1. PCR and Gel Electrophoresis Notes

2. PCR Put a sample of DNA in the thermocycler with free nucleotides and Taq polymerase Heat it up to 98 degrees Celsius This breaks the hydrogen bonds in the DNA double helix Cool it down to 62 degrees Free nucleotides complementary base pair Taq polymerase sticks them together Repeat 30x

3. Gel Electrophoresis Cut the DNA with Restriction Enzymes Inject the DNA fragments into wells in the gel Turn on an electric current DNA will be pulled through the gel matrix Fragments of the same size will get stuck in the same place forming bands that we can compare

4. Gel

5. Cloning