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Supplementary Figure 1. Summary of repeat field surveys and temporal genetic analysis for 10 sites with multiple years of collections, including (i) site occupancy histories from field surveys, tabulated on an annual basis (1=occupied, 0=unoccupied, * indicates year of genetic tissue collection), (ii) estimates of genetic diversity within each temporal genetic collection (N = sample size, H o = observed heterozygosity, H e = expected heterozygosity, A = allelic richness, A r = rarefied allelic richness, A p = rarefied private allelic richness), (iii) results from site-specific Bayesian cluster analysis using STRUCTURE assuming the data were composed of two genetically distinct clusters, (iv) Genetic differentiation (F ST ) between population pairs (below diagonal) and p-values from permutation tests for significance (above diagonal).
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Tillas Slough 1999 Site Occupancy: Field Surveys Genetic Diversity Bayesian Cluster Analysis (K=2)Genetic Differentiation (Pairwise F ST ) YearNHoHo HeHe AArAr ApAp 1999480.250.272.782.280 2011590.280.263.222.390 2011 19992011 1999 --0.559144 2011 -0.00164-- 1999200020012002200320042005200620072008200920102011 1*---01---1-1
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Lake Earl 19992006 Site Occupancy: Field Surveys Genetic Diversity Bayesian Cluster Analysis (K=2)Genetic Differentiation (Pairwise F ST ) YearNHoHo HeHe AArAr ApAp 1999470.250.244.222.880.11 2006490.270.2853.220.03 20101 2011540.250.283.892.90.03 20102011 199920062011 1999 --0.7583240.374163 2006 -0.00323--0.999300 2011 0.00052-0.00810-- 1999200020012002200320042005200620072008200920102011 1*---11- 11-
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Stone Lagoon 19902006 Site Occupancy: Field Surveys Genetic Diversity Bayesian Cluster Analysis (K=2)Genetic Differentiation (Pairwise F ST ) YearNHoHo HeHe AArAr ApAp 1990190.570.554.894.610.05 2006460.52 6.334.870.22 20101 20112 20102011 19902006 1990 --0.50335 2006 -0.00087-- 19 90 19 91 19 92 19 93 19 94 19 95 19 96 19 97 19 98 19 99 20 00 20 01 20 02 20 03 20 04 20 05 20 06 20 07 20 08 20 09 20 10 20 11 1*--1--11-1---11- 111
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Big Lagoon 2006 Site Occupancy: Field Surveys Genetic Diversity Bayesian Cluster Analysis (K=2)Genetic Differentiation (Pairwise F ST ) YearNHoHo HeHe AArAr ApAp 2006470.60.566.674.920.16 2010490.530.546.114.630.05 2011450.540.555.784.660.15 20102011 200620102011 2006 --0.0715930.770123 2010 0.00532--0.518748 2011 -0.00255-0.00065-- 200620072008200920102011 1*111
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McDaniel Slough 19992006 Site Occupancy: Field Surveys Genetic Diversity Bayesian Cluster Analysis (K=2)Genetic Differentiation (Pairwise F ST ) YearNHoHo HeHe AArAr ApAp 1999400.140.1621.770 2006310.18 1.781.70 2010120.20.191.78 2011590.14 1.781.480 20102011 199920062011 1999 --0.00020.144786 2006 0.07386--0.0001 2011 0.008050.06953-- 1999200020012002200320042005200620072008200920102011 1*---00- 00-
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Elk River Site Occupancy: Field Surveys Genetic Diversity Bayesian Cluster Analysis (K=2)Genetic Differentiation (Pairwise F ST ) YearNHoHo HeHe AArAr ApAp 2006420.27 21.840 20112 590.21 21.780 20062011 20062011 2006 --0.0001 2011 0.15022-- 200620072008200920102011 1*-111 2011
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Eel River 2006 Site Occupancy: Field Surveys Genetic Diversity Bayesian Cluster Analysis (K=2)Genetic Differentiation (Pairwise F ST ) YearNHoHo HeHe AArAr ApAp 2006450.270.32.782.550.02 2010470.25 2.672.270 2010 20062010 2006 --0.09599 2010 0.00736-- 20062007200820092010 1*---
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Virgin Creek 19881999 Site Occupancy: Field Surveys Genetic Diversity Bayesian Cluster Analysis (K=2)Genetic Differentiation (Pairwise F ST ) YearNHoHo HeHe AArAr ApAp 1988580.51 4.443.660.06 1999670.570.564.563.80.03 2006500.590.574.333.70.04 2010470.540.564.563.710.08 20062010 1988199920062010 1988 --0.0020.0001 1999 0.01091--0.0113990.0038 2006 0.034250.00817--0.522348 2010 0.037810.01091-0.00073-- 19 88 19 89 19 90 19 91 19 92 19 93 19 94 19 95 19 96 19 97 19 98 19 99 20 00 20 01 20 02 20 03 20 04 20 05 20 06 20 07 20 08 20 09 20 10 1*---------- ---1-- -1-
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Pudding Creek 1990 Site Occupancy: Field Surveys Genetic Diversity Bayesian Cluster Analysis (K=2)Genetic Differentiation (Pairwise F ST ) YearNHoHo HeHe AArAr ApAp 1990200.440.433.112.960 2006510.450.443.222.940 2010490.42 3.333.010 20062010 199020062010 1990 --0.1167880.921308 2006 0.00792--0.442856 2010 -0.00839-0.00005-- 19 90 19 91 19 92 19 93 19 94 19 95 19 96 19 97 19 98 19 99 20 00 20 01 20 02 20 03 20 04 20 05 20 06 20 07 20 08 20 09 20 10 1*-----1--1---1-- -1-
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Davis Lake 1990 Site Occupancy: Field Surveys Genetic Diversity Bayesian Cluster Analysis (K=2)Genetic Differentiation (Pairwise F ST ) YearNHoHo HeHe AArAr ApAp 1990200.410.4343.730.02 2013560.420.445.554.310.12 2013 19902013 1990 --0.178082 2013 0.00547-- 19 90 19 91 19 92 19 93 19 94 19 95 19 96 19 97 19 98 19 99 20 00 20 01 20 02 20 03 20 04 20 05 20 06 20 07 20 08 20 09 20 10 20 11 20 12 20 13 1*-----11-----1---------
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