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Structure of the SSB–DNA polymerase III interface and its role in DNA replication by Aimee H Marceau, Soon Bahng, Shawn C Massoni, Nicholas P George, Steven.

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Presentation on theme: "Structure of the SSB–DNA polymerase III interface and its role in DNA replication by Aimee H Marceau, Soon Bahng, Shawn C Massoni, Nicholas P George, Steven."— Presentation transcript:

1 Structure of the SSB–DNA polymerase III interface and its role in DNA replication by Aimee H Marceau, Soon Bahng, Shawn C Massoni, Nicholas P George, Steven J Sandler, Kenneth J Marians, and James L Keck EMBO J. Volume 30(20):4236-4247 October 19, 2011 ©2011 by European Molecular Biology Organization

2 Structure of the E. coli χ/ψ/SSB ‐ Ct complex. Aimee H Marceau et al. EMBO J. 2011;30:4236-4247 ©2011 by European Molecular Biology Organization

3 Identification of χ variants with compromised SSB binding. Aimee H Marceau et al. EMBO J. 2011;30:4236-4247 ©2011 by European Molecular Biology Organization

4 Conditional rolling ‐ circle DNA replication by R128A and R128E/K132E χ Pol III HE. Rolling ‐ circle DNA replication reactions (schematically outlined in (A)) were catalysed by wt, R128A χ or R128E/K132E Pol III HE in the absence of added salt (B–D) or with 70... Aimee H Marceau et al. EMBO J. 2011;30:4236-4247 ©2011 by European Molecular Biology Organization

5 Growth and DNA replication in E. coli holC ‐ mutant strains. Aimee H Marceau et al. EMBO J. 2011;30:4236-4247 ©2011 by European Molecular Biology Organization

6 Filamentation and SOS induction in E. coli holC ‐ mutant strains. Aimee H Marceau et al. EMBO J. 2011;30:4236-4247 ©2011 by European Molecular Biology Organization


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