2. Mosquitoes Bite How to genetically engineer the Anopheles mosquito to be resistant to Plasmodium

3. Plasmodium Parasite that causes Malaria

4. Anopheles Life Cycle

5. Cellular Target 2 places –Salivary Gland (parasite exits) –Gut (parasite grows)

6. Immunity Gene Scientists have found multiple genes in mosquitoes that make it resistant to Plasmodium One of these is the Rel 2 gene which is located in the gut of the mosquito It is deactivated by the Caspar gene

7. Isolating the Gene 2 possible methods –Reverse Transcriptase –Polymerase Chain Reaction …is a DNA polymerase enzyme that transcribes single-stranded RNA into double-stranded DNA. PCR Video

8. Construction Plasmid Immune Gene (Isolated with Restriction Enzymes) Green Gene New Plasmid!

9. Insertion Microinjection. Zygote Genetically engineered plasmid with Rel 2 gene and green gene