1. MBV2010 Colloquium, February 25

2. RULES Multiple choice Only one correct answer 30-60 seconds to answer No textbook, computer, mobile phone, please There will be a prize for the group with the most correct answers.

3. Example (does not count): What is astraphobia ? A Fear of lightning B Fear of spiders C Fear of heights D Fear of snakes 0

4. What is astraphobia ? 0

5. Which of the following systems repairs DNA mismatches? A RecBCD B MutSLH C UvrABC D DNA glycosylases 1

6. Which of the following systems repairs DNA mismatches? 1

7. What is shown in this figure? A histone modifications in telomeres B histone modifications in protein-coding genes C replacement of histone H3 in centromeres D replacement of histone H2A 2

8. What is shown in this figure? 2

9. What is the role of the SeqA protein in E. coli ? 3 A initiate replication B prevent replication C break hydrogen bonds D relieve topological stress in DNA

10. What is the role of the SeqA protein in E. coli ? 3 SeqA SeqA binds to hemimethylated DNA CH 3

11. When in a cell cycle is the eukaryotic origin recognition complex bound to DNA? 4 A in G 1, S, and G 2 B in S, G 2, and M C in G 2, M, and G 1 D only at the end of G 1

12. When in a cell cycle is the eukaryotic origin recognition complex bound to DNA? 4

13. Which of the following proteins is involved in initiation of replication in the nucleus? 5 A DnaA B Type II topoisomerases C Cdc6 D Cdc45

14. Which of the following proteins is involved in initiation of replication in the nucleus? 5

15. Replication protein A (RPA) is a 6 A helicase B topoisomerase C single-strand binding protein D DNA polymerase

16. Replication protein A (RPA) is a 6

17. The proliferating cell nuclear antigen (PCNA) is involved in 7 A cell division B nucleosome assembly C genome replication D formation of heterochromatin

18. The proliferating cell nuclear antigen (PCNA) is involved in 7

19. Which of the following DNA polymerases synthesizes DNA in a template-independent manner? A DNA polymerase I B DNA polymerase III C DNA polymerase IV D DNA polymerase ß 8

20. Which of the following DNA polymerases synthesizes DNA in a template-independent manner? 8

21. Which of the following sequences is complementary to 5’-AATGAA-3’ ? A 5’-TTACTT-3’ B 5’-AACTAA-3’ C 5’-TTCATT-3’ D 5’-AAGTAA-3’ 9

22. Which of the following sequences is complementary to 5’-AATGAA-3’ ? 9

23. Which proteins are shown in this figure? 10 A RuvA (1) and RuvB (2) B RuvA (2) and RuvB (1) C RuvA (1) and RuvC (2) D RuvB (1) and RuvC (2) 1 2

24. Which proteins are shown in this figure? 10 1 2

25. SWI/SNF is a A histone modification complex B nucleosome remodeling complex C nucleosome assembly complex D none of the above 11

26. SWI/SNF is a 11

27. Which of the following proteins resolves Holliday junctions? A RuvA B RuvB C RecA D RuvC 12

28. Which of the following proteins resolves Holliday junctions? 12 RuvC

29. The yellow protein in this figure is 13 A a DNA topoisomerase B a DNA polymerase C a DNA helicase D a primase

30. The yellow protein in this figure is 13