1. Date of download: 6/23/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Increased Angiogenic Factors Associated With Peripheral Avascular Retina and Intravitreous Neovascularization: A Model of Retinopathy of Prematurity Arch Ophthalmol. 2010;128(5):589-595. doi:10.1001/archophthalmol.2010.65 Lectin-stained retinal flat mount from a rat pup at postnatal age 14 days in the 50/10 oxygen–induced rat model of retinopathy of prematurity following 7 cycles of oxygen fluctuations every 24 hours between 50% oxygen and 10% oxygen. Peripheral avascular retina persists, whereas room air–raised rat pup counterparts have fully vascularized retina. Figure Legend:

2. Date of download: 6/23/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Increased Angiogenic Factors Associated With Peripheral Avascular Retina and Intravitreous Neovascularization: A Model of Retinopathy of Prematurity Arch Ophthalmol. 2010;128(5):589-595. doi:10.1001/archophthalmol.2010.65 Lectin-stained retinal flat mount from a rat pup at postnatal age 18 days in the 50/10 oxygen–induced rat model of retinopathy of prematurity (7 cycles of oxygen fluctuations every 24 hours between 50% oxygen and 10% oxygen, followed by 4 days in room air). Intravitreous neovascularization exists at the junction of vascular and avascular retina. Figure Legend:

3. Date of download: 6/23/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Increased Angiogenic Factors Associated With Peripheral Avascular Retina and Intravitreous Neovascularization: A Model of Retinopathy of Prematurity Arch Ophthalmol. 2010;128(5):589-595. doi:10.1001/archophthalmol.2010.65 Fold change in isoform vascular endothelial growth factor 164 (VEGF 164 ) messenger RNA (mRNA) expression, relative to β-actin, in room air–raised rat pups (RA) and the 50/10 oxygen–induced rat model of retinopathy of prematurity (50/10 OIR model). Significantly increased fold change in VEGF 164 mRNA expression was present in the 50/10 OIR model compared with RA at 14 days (*P <.001) and at 18 days (**P =.02) (t test). Figure Legend:

4. Date of download: 6/23/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Increased Angiogenic Factors Associated With Peripheral Avascular Retina and Intravitreous Neovascularization: A Model of Retinopathy of Prematurity Arch Ophthalmol. 2010;128(5):589-595. doi:10.1001/archophthalmol.2010.65 Fold change in vascular endothelial growth factor receptor 2 (VEGFR2) messenger RNA (mRNA) expression, relative to β-actin, in room air–raised rat pups (RA) and the 50/10 oxygen–induced rat model of retinopathy of prematurity (50/10 OIR model). Significantly increased fold change in VEGFR2 mRNA expression was present in 50/10 OIR compared with RA at 18 days (*P =.004). A trend toward increased fold change in VEGFR2 mRNA expression was noted in the 50/10 OIR model at 14 days (P =.06) (t test). Figure Legend:

5. Date of download: 6/23/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Increased Angiogenic Factors Associated With Peripheral Avascular Retina and Intravitreous Neovascularization: A Model of Retinopathy of Prematurity Arch Ophthalmol. 2010;128(5):589-595. doi:10.1001/archophthalmol.2010.65 Fold change in vascular endothelial growth factor receptor (VEGFR1) messenger RNA (mRNA) expression, relative to β-actin, in room air–raised rat pups (RA) and the 50/10 oxygen–induced rat model of retinopathy of prematurity (50/10 OIR model). No significant differences were found at 14 days (P =.47) or at 18 days (P =.97) (t test). Figure Legend:

6. Date of download: 6/23/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Increased Angiogenic Factors Associated With Peripheral Avascular Retina and Intravitreous Neovascularization: A Model of Retinopathy of Prematurity Arch Ophthalmol. 2010;128(5):589-595. doi:10.1001/archophthalmol.2010.65 Fold change in neuropilin 1 (NP1) messenger RNA (mRNA) expression, relative to β-actin, in room air–raised rat pups (RA) and the 50/10 oxygen–induced rat model of retinopathy of prematurity (50/10 OIR model). Significantly increased fold change in NP1 mRNA expression was present in the 50/10 OIR model compared with RA at 14 days (*P =.04) and at 18 days (**P =.03) (t test). Figure Legend:

7. Date of download: 6/23/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Increased Angiogenic Factors Associated With Peripheral Avascular Retina and Intravitreous Neovascularization: A Model of Retinopathy of Prematurity Arch Ophthalmol. 2010;128(5):589-595. doi:10.1001/archophthalmol.2010.65 Fold change in neuropilin 2 (NP2) messenger RNA (mRNA) expression, relative to β-actin, in room air–raised rat pups (RA) and the 50/10 oxygen–induced rat model of retinopathy of prematurity (50/10 OIR model). Significantly increased fold change in NP2 mRNA expression was present in the 50/10 OIR model compared with RA at 14 days (*P =.001) and at 18 days (**P =.002) (t test). Figure Legend:

8. Date of download: 6/23/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Increased Angiogenic Factors Associated With Peripheral Avascular Retina and Intravitreous Neovascularization: A Model of Retinopathy of Prematurity Arch Ophthalmol. 2010;128(5):589-595. doi:10.1001/archophthalmol.2010.65 Fold change in pigment epithelium–derived factor (PEDF) messenger RNA (mRNA) expression, relative to β-actin, in room air– raised rat pups (RA) and the 50/10 oxygen–induced rat model of retinopathy of prematurity (50/10 OIR model). No significant difference was found at 14 days (P =.47) or at 18 days (P =.39) (t test). Figure Legend:

9. Date of download: 6/23/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Increased Angiogenic Factors Associated With Peripheral Avascular Retina and Intravitreous Neovascularization: A Model of Retinopathy of Prematurity Arch Ophthalmol. 2010;128(5):589-595. doi:10.1001/archophthalmol.2010.65 Fold change in pigment epithelium–derived factor receptor (PEDFR) messenger RNA (mRNA) expression, relative to β-actin, in room air–raised rat pups (RA) and the 50/10 oxygen–induced rat model of retinopathy of prematurity (50/10 OIR model). Significantly increased fold change in PEDFR mRNA expression was present in the 50/10 OIR model compared with RA at 14 days (*P <.001). No significant difference was found at 18 days (P =.32) (t test). Figure Legend: