1. Naturalized Dyes Synthesis Serichim Activities from February 2016 to May 2016 Serichim, 11/02/20161 Project LIFE12 ENV/IT/352 "Naturalised dyes replacing commercial colorants for environmentally friendly leather dyeing and water recycle" (BIONAD)

2. Support to Life Cycle Analysis of Naturalized Dyes: manufacturing step Elements to be collected and evaluated: Raw materials, solvents and auxiliaries involved in synthesis Liquid and solid wastes as a function of their final fate Emissions Utility types and consumptions

3. Estimation criteria for chemicals Raw materials Solvents Auxiliaries Derived from experimental specific consumptions Estimated as a percentage (90%) of experimental specific consumption, in view of a partial recycle. Derived from experimental specific consumptions, without recovery and recycle

4. Estimation criteria for wastes Estimated from material balances, grouping effluents according to their chemical nature: Organic wastes (directly from material balances): Chlorinated waste Organic water insoluble waste, not chlorinated Aqueous wastes: High COD waste: water soluble organic waste, diluted to have a stream at 500 ppm of COD High salt content waste (up to 20% in case of brine) High HCl content waste

5. Utilities Type only listed, not specific consumptions: Steam Hot water Industrial water Cooling water Chilled water Nitrogen Power

6. How the analysis is structured: block organization Lactose Derivate Yellow Derivate Orange Derivate Blue Derivate Yellow NAT Orange NAT Blue NAT Lactose 3 STEPS Commercial Dyes 2 STEPS