1. Minnesota First Detectors EAB or Not? A. Yes B. Not C. Not Sure

2. Minnesota First Detectors EAB or Not? A. Yes B. Not C. Not Sure

3. Minnesota First Detectors EAB or Not? A. Yes B. Not C. Not Sure

4. Minnesota First Detectors EAB or Not? A. Yes B. Not C. Not Sure

5. Minnesota First Detectors EAB or Not? A. Yes B. Not C. Not Sure

6. Minnesota First Detectors EAB Distribution

7. Minnesota First Detectors EAB Distribution 1.31.2014

8. Minnesota First Detectors EAB Distribution

9. Minnesota First Detectors EAB Distribution

10. Minnesota First Detectors EAB Risk from Iverson, et al. 2013

11. Minnesota First Detectors Winter Update

12. Minnesota First Detectors Cold Mortality Predicting Mortality is Tricky Larval freeze temperature varies Temperatures within trees varies

13. Minnesota First Detectors (Map by R.C. Venette) January 2014 Intl Falls-39F Superior-23F Twin Cities -23°F La Crosse -20F

14. Minnesota First Detectors Winter mortality and EAB dynamics Ash mortality Seasonal growth 60% winter mortality 90% winter mortality “Compound interest”

15. Minnesota First Detectors

17. Biological control Wasp releases Larval parasitoid Egg parasitoid Larval parasitoid recovery in SE MN Attacking EAB Reproducing Dispersing (found a half mile from nearest release)

18. Minnesota First Detectors Larval parasitoid, Tetrastichus planipennisi J. Lelito, USDA APHIS

19. Minnesota First Detectors Egg parasitoid, Oobius agrili

20. Minnesota First Detectors Biosurveillance Smoky winged beetle bandit Native wasp Attacks wood-boring beetles including EAB Collect beetles from wasps Abandoned beetles Catch and release wasps Photo: Michael Bohne, US Forest Service Photo: Connecticut Agricultural Experiment Station

21. Minnesota First Detectors Questions

22. Minnesota First Detectors Larval parasitoid, Spathius agrili