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(a)(b) Fig. S1 Figure S1. Functional classification of genes that were upregulated (a) or downregulated (b) by systemic application of GA. Five micro liter.

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Presentation on theme: "(a)(b) Fig. S1 Figure S1. Functional classification of genes that were upregulated (a) or downregulated (b) by systemic application of GA. Five micro liter."— Presentation transcript:

1 (a)(b) Fig. S1 Figure S1. Functional classification of genes that were upregulated (a) or downregulated (b) by systemic application of GA. Five micro liter of 10 –6 M GA 4 was applied to the shoots of GA-deficient mutant plants, and roots were collected after three days for microarray analysis. Genes were classified as differentially expressed when signal values deviated by ≥4-fold.

2 Fig. S2 (a) (b) Figure S2. Root and shoot growth in GA-deficient mutant plants following application of CLE6 to shoots. Shoots of 3-week-old GA-deficient plants were treated with 0.1 to 0.75  M synthetic CLE6 peptide. (a) Number of leaves. (b) Primary root length. Error bars represent standard error (n = 40 plants).

3 Fig. S3 (a) (b) (c) Figure S3. CLE6 mRNA levels and root phenotypes in CLE6-overexpressing GA- deficient lines. (a) Assessment of CLE6 mRNA abundances using real-time PCR was performed in roots of 3-week-old plants. (b) Primary root lengths and (c) average lateral root lengths in CLE6-overexpressing ga3ox1/ga3ox2 compared with wild-type (WT) and ga3ox1/ga3ox2. Error bars represent standard error (n = 40 plants). 35S::CLE6#1 was used for subsequent experiments.

4 Figure S4. Levels of CLE6 mRNA in ga3ox1/ga3ox2 roots cultured without GA application. Data are means of triplicate experiments ± SD. Growth condition is same as described in Figure 3. Fig. S4


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