1. Performing HIV Rapid Tests
2005
Performing HIV Rapid Tests
Demonstration and Practice
Module 9: Performing HIV Rapid Tests

2. Learning Objectives At the end of this module, you will be able to:
2005
Learning Objectives
At the end of this module, you will be able to:
Perform 3 HIV rapid tests according to SOP
KHB Rapid HIV 1/2
Stat-Pak HIV1/2
Uni-Gold HIV1/2
Perform multiple tests simultaneously
Accurately interpret individual test results
Accurately determine HIV status
Module 9: Performing HIV Rapid Tests

3. Optional hands-on session
Content Overview
2005
Overview of Testing Procedures
Workspace Setup
Practice
(Multi-test algorithm with
blind samples)
Demo + Practice
(individual tests on
known samples)
Optional hands-on session
Module 9: Performing HIV Rapid Tests

4. 2005
KHB rapid
Module 9: Performing HIV Rapid Tests

5. Make ready the device and label with code number
123
Test line
Control Line
Sample port 5 5

6. KHB: Specimen collection6

7. Add 40 l of whole/plasma/serum7

8. Add 1 drop of running buffer
123 8 8

9. Test interpretation (within 30 minutes)
Positive Test
123 9

10. Test interpretation
Negative Test
123 10

11. Test interpretation
Invalid result
123 11

12. The whole procedure (30’)12

13. STAT-PAK

14. STAT-PAK : Getting Ready
2. Remove device from package, place it on flat surface and label device with client identification number
1. Collect test items and other necessary laboratory supplies .

15. STAT-PAK: Collecting Specimen
3. Fill the 5µl loop provided with the kit with whole blood, plasma or serum

16. STAT-PAK: Adding Specimen and Reagent to Test Device
4. Holding the sample loop vertically apply the 5µl of sample to the Sample pad (S)
5. Add 3 drops (150 µl) buffer slowly, drop wise into the Sample (S) well

17. STAT-PAK: Getting Results
6. Read the results between 15 and 20 minutes after addition of running buffer
7. Record results and other pertinent information on the worksheet

18. Stat-Pak: Test Interpretation
Non-reactive Reactive Invalid

19. 2005
Uni-Gold
Module 9: Performing HIV Rapid Tests

20. Uni-Gold: Getting Ready
2005
Uni-Gold: Getting Ready
2. Remove device from package
and label device with client
identification number
1. Collect test items and other
necessary lab supplies
Module 9: Performing HIV Rapid Tests

21. Uni-Gold: Collecting Specimen
2005
Uni-Gold: Collecting Specimen
3. Collect specimen using the disposable pipette
Module 9: Performing HIV Rapid Tests

22. Uni-Gold: Adding Specimen and Reagent to Test Device
2005
Uni-Gold: Adding Specimen and Reagent to Test Device
4. Add 2 drops (approx. 60µl) of
specimen to the sample port in the device
5. Add 2 drops (approx. 60µl) of the
appropriate wash reagent to sample
port
Module 9: Performing HIV Rapid Tests

23. Uni-Gold: Getting Results
2005
Uni-Gold: Getting Results
6. Wait for 10 minutes (no longer than
20 min.) before reading the results
7. Read and record the results and
other pertinent info on the worksheet
Module 9: Performing HIV Rapid Tests

24. Uni-Gold: Test Interpretation
2005
Uni-Gold: Test Interpretation
Reactive
Non-reactive
Invalid
Module 9: Performing HIV Rapid Tests

25. Activity: Workspace Setup
2005
Activity: Workspace Setup
Instructions:
Identify your Workspace in the practical room
Gather test kits and supplies
Obtain reactive and non-reactive specimens from instructor
Arrange all items at your work station
Activity time: 10 minutes
Module 9: Performing HIV Rapid Tests

26. Hands-On Practice: Individual Tests
2005
Hands-On Practice: Individual Tests
Instructions:
Use safety precautions
Practice with blood provided by your instructor only
Raise your hand if you need additional supplies
Show your test results to instructor after you are done
Total time: 15 minutes per test
Module 9: Performing HIV Rapid Tests

27. National Algorithm: Ethiopia
2005
Blood Sample
Test 1 (KHB rapid )
Reactive
Non-reactive
Report Negative
Test 2 (Stat Pak)
Non Reactive
Reactive
Report Positive
Test 3 (Unigold)
Reactive Result
Report Positive
Non-reactive Result
Report Negative
Lab workers
Health workers
Counselors
Module 9: Performing HIV Rapid Tests

28. Possible Outcomes in a serial Algorithm
2005
Possible Outcomes in a serial Algorithm
KHB rapid
Stat-Pak
Uni-Gold
HIV Status
Non-reactive
Negative
Reactive
Positive
Invalid test needs to be repeated
Does not count toward outcome of test
Lab workers
Health workers
Counselors
Module 9: Performing HIV Rapid Tests

29. Hands-On Practice: Multi-Test Algorithm
2005
Hands-On Practice: Multi-Test Algorithm
Determine the HIV status for each of the three blind specimens provided by following your country’s serial algorithm 1
Test 1 - Test 2 - Test 3
HIV Status 2 3 4 5
Specimen
Module 9: Performing HIV Rapid Tests

30. Hands-On Practice: Multi-Test Algorithm (Cont’d)
2005
Hands-On Practice: Multi-Test Algorithm (Cont’d)
Instructions:
Collect supplies and obtain panel of blind specimens
Organize your workspace
Complete one algorithm before starting the next
Don’t forget – Use safety precautions
Practice on blood provided by your instructor only
Raise your hands if you need additional supplies
Record results on worksheet
Keep test devices - instructor will check results before discarding of devices
Total time: 2 hours
Module 9: Performing HIV Rapid Tests

31. Summary Describe the key learning from performing:
2005
Summary
Describe the key learning from performing:
KHB rapid HIV 1/2
Stat-Pak HIV 1/2
Uni-Gold HIV ½
Describe the key learning from performing the multi-test algorithm
Module 9: Performing HIV Rapid Tests