1. NICOTINE and the BRAIN Wilson M. Compton, MD, MPE Deputy Director, U.S. National Institute on Drug Abuse

2. Tobacco is addictive because of the rewarding/reinforcing effects of nicotine (both positive and negative reward). Development is important. Adolescent exposure may be key to addiction. Electronic nicotine delivery systems (“E- cigarettes”) have both promise and concern. Summary

3. All Drugs Abused by Humans Raise Brain Dopamine Levels in the Nucleus Accumbens Nestler, Nature Neurosci, 2005 ` ` Time After Methamphetamine Dopamine (nM) METHAMPHETAMINE 2000 1500 1000 500 0 2000 1500 1000 500 0 5 5 1 1 2.5 Dose (mg/kg IV) 0 20 40 60 80min 0 100 150 200 250 0 123 hr Time After Nicotine % of Basal Release NICOTINE Di Chiara et al.

4. TYROSINE DA DOPA DA DA DA DA TYROSINE DA DOPA DA DA DA DA DA DA DA DA DA DA methylphenida te R R R R R R -10010203040 -2 0 2 4 6 8 10 Self-Reports (0-10) Change in Dopamine Bmax/kd (Placebo - MP) “High” Increases in Dopamine are Associated with Perceived “High” raclopride DA Volkow et al., JPET 291(1):409-415, 1999. NAcc VP REWARD

5. DARK SIDE OF ADDICTION

6. Biological Psychiatry Convergent Results Support CHRNA5/A3/B4 Gene Cluster Association with Nicotine DependenceMedialhabenula a5, a3,  4, have a high concentration in habenula and interpeduncular nucleus

7. Kimura et al., Nature Neuroscience 2007. Habenula neurons increased firing for NO REWARD and decreased firing for REWARD Dopamine neurons increased firing for REWARD and decreased firing for NO REWARD. HABENULA Habenula inhibits dopamine neurons Habenula communicates negative reward signals to dopamine neurons Habenula and Negative Reward

8. ACG OFC SCC Hipp NAcc VP Amyg REWARD INHIBITORY CONTROL INHIBITORY CONTROL MEMORY/ LEARNING MEMORY/ LEARNING EXECUTIVE FUNCTION EXECUTIVE FUNCTION PFC Becomes severely disrupted in ADDICTION MOTIVATION/ DRIVE MOTIVATION/ DRIVE The fine balance in connections that normally exists between brain areas active in reward, motivation, learning and memory, and inhibitory control

9. Tobacco is addictive because of the rewarding/reinforcing effects of nicotine (both positive and negative reward). Development is important. Adolescent exposure may be key to addiction. Electronic nicotine delivery systems (“E- cigarettes”) have both promise and concern. Summary

10. CIGARETTES ALCOHOL MARIJUANA COCAINE HEROIN NONE Nicotine as a Gateway Drug Pathways of Drug Involvement Most cocaine users have smoked (NSDUH 2009) Source: Denise Kandel

11. Source: Levine A. et al., Sci Transl Med. 2011.

12. Tobacco is addictive because of the rewarding/reinforcing effects of nicotine (both positive and negative reward). Development is important. Adolescent exposure may be key to addiction. Electronic nicotine delivery systems (“E- cigarettes”) have both promise and concern. Summary

13. Electronic Nicotine Delivery Systems: “E-cigarettes” Slide Adapted from and Courtesy of Thomas Eissenberg, Virginia Commenwealth University, USA

14. Electronic Nicotine Delivery Systems: “E-cigarettes” Slide Adapted from and Courtesy of Thomas Eissenberg, Virginia Commenwealth University, USA

15. Device design influences nicotine yield… Data from Talih et al., under review. Slide Courtesy of Thomas Eissenberg, Virginia Commenwealth University, USA

16. Some Devices Do Not Deliver Nicotine to Naïve Users Vansickel et al., 2010 Note: 32 ECIG naïve cigarette smokers; 2, 10 puff bouts Tobacco cigarette Slide Courtesy of Thomas Eissenberg, Virginia Commenwealth University, USA

17. Data from Vansickel and Eissenberg, 2013 + additional participants. BUT Some Devices Deliver Nicotine to Experienced Users 0 5 10 15 20 25 Time (minutes) 10-PuffAd-Lib ng/ml Slide Courtesy of Thomas Eissenberg, Virginia Commenwealth University, USA

18. E-Cig Users Take Larger, Longer, Slower Puffs Tobacco cigarette data from Kleykamp et al., 2008. See also Farsalinos et al., 2013 (video recording of use) and Hua et al., 2012 (YouTube analysis). Slide Courtesy of Thomas Eissenberg, Virginia Commenwealth University, USA

19. 19 Understanding Topography is Relevant to Health Slide Courtesy of Thomas Eissenberg, Virginia Commenwealth University, USA

20. Electronic Nicotine Delivery Systems: Promise or Peril? Promise: Promise: “If governments, parliaments, regulation agencies, and experts are able to restrain their yearning to restrict access to e-cigarettes, these products are likely to represent a revolution in public health.” Etter, 2013. Peril: Peril: “Urged on by myopic health professionals who seem to have lost any population health focus they might have had, this may become one of the biggest blunders of modern public health.” Chapman, 2013. Middle ground: Middle ground: “… [our responses to ECIGs] will provide the greatest public health benefit when they are proportional, based on evidence, and incorporate a rational appraisal of likely risks and benefits.” Hajek et al., in press. Slide Courtesy of Thomas Eissenberg, Virginia Commenwealth University, USA

21. Science is Essential to Inform Public Health Tobacco is addictive because of the rewarding/reinforcing effects of nicotine (both positive and negative reward). Development is important. Adolescent exposure may be key to addiction. Electronic nicotine delivery systems (“E- cigarettes”) have both promise and concern.