1. Egg lab day 1

2. ‘Diffusion Demonstration’ In journal, make a title and draw the two flasks as shown below. START FINISH

3. Vocabulary (in your Egg Lab Booklet, page 7) Solution: a mixture of two or more substances that are evenly distributed. Solute: the substance that gets dissolved in a liquid solution. Solvent: the substance that does the dissolving in a liquid solution.

4. Example In the ocean, the water is the solvent and the salt is the solute.

5. What is the solute and solvent in each example solution (write in journal): Kool-Aid Solvent: Solute(s): Hot Chocolate Solvent: Solute(s):

6. What is the solute and solvent in each example solution (write in journal): Kool-Aid Solvent: water Solute(s): flavor, sugar Hot Chocolate Solvent: milk (or water) Solute(s): chocolate flavor powder, sugar

7. Demonstration- part II Now draw the finish diagram and describe in words what happened from beginning to end START FINISH

8. More Vocabulary Diffusion: the movement of substances from high concentrations to low concentrations until an equilibrium is reached. (CO 2, O 2 ) Osmosis: the diffusion of water (from high concentrations to lower concentrations) through a selectively permeable membrane (until an equilibrium is reached).

9. Egg lab day 2 Yeah…the shell is gone! Now we can get at the cell membrane.

10. Beaker A: 200 mL water 20 mL food color Beaker B: 200 mL water 40 mL food color Make a title in your journal and copy down the pictures and labels below. Comparing Concentrations

11. Beaker A: 200 mL water 20 mL food color Beaker B: 200 mL water 40 mL food color Please answer the following questions in complete sentences. 1.Which beaker has more water? 2.Which beaker has more food color? 3.Which beaker has a greater concentration of food color? 4.Which beaker has a greater concentration of water?

12. More practice: please copy down the example below in your journal. Quick sketches are fine! Be sure to label with amounts of tea and sugar for each. Cup A: 12 oz of tea 1 oz sugar Cup B: 8 oz of tea 1 oz sugar

13. Finish Each: 1. Cup _____ has more tea (solvent) 2. Cup _____ has more sugar (solutes) 3. Cup _____ has a greater concentration of solutes. 4. Cup _____ has a greater concentration of solvent. Cup A: 12 oz of tea 1 oz sugar Cup B: 8 oz of tea 1 oz sugar

14. Name: ______________ Formative Quiz: Pitcher A: 1 liter water 100 mL lemon juice 1.Which pitcher has more water? 2.Which pitcher has more lemon juice? 3.Which pitcher has a higher concentration of lemon juice? 4.Which pitcher has a high concentration of water? Pitcher B: 1 liter water 200 mL lemon juice

15. Why does solution concentration matter? The solvent is represented by the pink liquid. The solutes are the small circles. Which moves through the membrane…the solvent or the solutes? Compare/contrast the concentration of the solvent before and after.

16. The red blood cells are very important to survival because they transport oxygen from lungs to the cells of the various tissues (muscles, nerves, etc.). These blood cells are transported in a fluid (serum) that has approximately the same salt content as sea water. If someone were admitted to the hospital and was in need of an IV (intravenous fluids) why is it critical that the concentrations of solutes (i.e. salts) in the IV is similar to that in the blood stream? 1.What would happen to the blood cells if the IV were too salty? 2.What would happen to the blood cells if the IV was pure water (less salty than water)? OSMOSIS AND BLOOD CELLS

17. Blood Cells in different solutions Dehydrated Cells: water leaves cells because the solution that cells are in is too salty. Bloated Cells: water enters cells because the solution that cells are in is not salty enough. Happy Cells: concentration of salts equal in and out of cells.

18. End day 2 STOP

19. Electrolyte is a "medical/scientific" term for salts, specifically ions. The term electrolyte means that this ion is electrically-charged and moves to either a negative (cathode)or positive (anode) electrode: ions that move to the cathode (cations) are positively charged ions that move to the anode (anions) are negatively charged For example, your body fluids -- blood, plasma, interstitial fluid (fluid between cells) -- are like seawater and have a high concentration of sodium chloride (table salt, or NaCl). The electrolytes in sodium chloride are: sodium ion (Na + ) - cation chloride ion (Cl - ) - anion As for your body, the major electrolytes are as follows: sodium (Na + ) potassium (K + ) chloride (Cl - ) calcium (Ca 2+ ) magnesium (Mg 2+ ) bicarbonate (HCO 3 - ) phosphate (PO 4 2- ) sulfate (SO 4 2- ) Electrolytes are important because they are what your cells (especially nerve, heart, muscle) use to maintain voltages across their cell membranes and to carry electrical impulses (nerve impulses, muscle contractions) across themselves and to other cells. Your kidneys work to keep the electrolyte concentrations in your blood constant despite changes in your body. For example, when you exercise heavily, you lose electrolytes in your sweat, particularly sodium and potassium. These electrolytes must be replaced to keep the electrolyte concentrations of your body fluids constant. So, many sports drinks have sodium chloride or potassium chloride added to them. They also have sugar and flavorings to provide your body with extra energy and to make the drink taste better.cellskidneysexercisesweat Another example where electrolyte drinks are important is when infants/children have chronic vomiting or diarrhea, perhaps due to intestinal flu viruses. When children vomit or have diarrhea, they lose electrolytes. Again, these electrolytes and the fluids must be replaced to prevent dehydration and seizures. Therefore, drinks such as Pedialyte have sodium and potassium in them like the sports drinks do. However, pediatricians do not recommend giving sports drinks to a sick child! Sports drinks have much higher sugar concentrations than Pedialyte and the high sugar is not a proper treatment.flu viruses

20. Day 3:Slides 19-27

21. Egg lab day 3 Follow directions for DAY THREE in your booklet to collect/record data.

22. Making sense of the Egg Lab Did the egg placed in pure water change during this lab?

23. WHY did the egg in pure water get bigger? Hint: remember only water can pass through the egg’s membrane.

24. The egg in pure water got bigger because: Since water is the only substance that can go through the egg membrane, water entered the egg (the egg absorbed water) But WHY did water enter the egg?

25. WHY did water enter the egg? Water always moves from HIGH CONCENTRATIONS to LOW CONCENTRATIONS during osmosis. Since the concentration of water was highest outside the egg (pure water = 100 % water), water enters the egg (which was 90% water).

26. What happened to the egg placed in corn syrup?

27. Since water is the only substance that can go through the egg membrane, water left the egg (and went into the corn syrup solution). But why did water leave the egg? Why did the egg in corn syrup get smaller(less mass)? The egg in corn syrup got smaller because:

28. Corn syrup is about 25% water and the egg is about 90% water. Water always move from high concentration to low concentration…so water diffused through the egg membrane and entered the corn syrup solution – OSMOSIS! WHY did water leave the egg?

29. Finish conclusions – pages 5+6

30. After the egg lab: slides 29-36 Definitions Endo/exo Definitions Group task – graphic organizer

31. Diffusion and osmosis works for some molecules. What happens if the molecules needed by the cell can’t pass directly through the cell membrane (lipid bi-layer)?

32. Definitions Facilitated Diffusion: the diffusion of larger molecules through special channel proteins in the cell membrane. (sodium, potassium, glucose)

33. Diffusion, osmosis, facilitated diffusion are all types of … Passive Transport: Transportation of materials in and out of cells using diffusion or osmosis; does not require the cell to use energy.

34. Endocytosis Model: How do cells get really BIG molecules in or out of the cells? Materials Plastic Bag (cell membrane) Candy (large ‘food’ molecules) String Scissors Problem: The bag at your table represents a cell membrane. The cell really wants the food molecules (candy), but they are too big to pass through the membrane.

35. Endocytosis Model: How do cells get really BIG molecules in or out of the cells? Your job is to figure out a way for the cell to get the food molecules (candy), without ever exposing the inside of the cell to the outside world or without ever having an OPEN HOLE in the bag. Remember that the cell is filled with cytoplasm (liquid). When getting the food molecules there should never be an opportunity for any cytoplasm liquid to escape. Good luck! The first group to successfully solve this problem gets an additional food molecule (candy)!

36. Cell absorbs a large amount of material

37. Exocytosis Cell releases a large amount of material.

38. Definitions Active Transport: When energy is required to transport materials in or out of cells. When a cell uses energy to obtain or get rid of something it needs. For example, it would take energy to move materials against diffusion.

39. Definitions Endocytosis/Exocytosis: transport of large molecules Into the cell - endocytosis. (glucose) Out of the cell - exocytosis. (waste, proteins) Examples of Active Transport: Molecular Transport (Pumps): Protein molecules in the cell membrane act like pumps and work against the forces of diffusion. Examples: calcium, potassium, or sodium.

40. Group/Individual Task: Complete the chart using your notes. Type of Movement Energy Required? Passive or Active Transport? What type of particle is moved? What part of the membrane is the particle moving through? How does it work? Explain (show picture) Diffusion Osmosis Facilitated Diffusion Molecular Transport Endocytosis Exocytosis

41. Type of Movement Energy Required? Passive or Active Transport? What type of particle is moved? What part of the membrane is the particle moving through? How does it work? Explain Diffusion No energy needed. Passive Transport O2 CO2 Lipid bi-layerParticles move through the lipid bi- layer until the concentrations in/out of the cell are balanced (equilibrium). Osmosis No energy needed. Passive Transport H20Lipid bi-layerWater moves through the lipid bi- layer until concentrations in/out of the cell are at equilibrium. Facilitated Diffusion No energy needed. Passive transport Glucose, sodium, potassium, calcium Protein channel Particles move from high to low concentrations through the protein channel. Molecular Transport Energy needed. Active transport Sodium, calcium, potassium Protein pumpThe cell uses energy to pump the particles against the concentration (from low to high!). Endocytosis Energy needed. Active transport Large molecules or clumps of material such as glucose. The whole membrane. The particle(s) are too big to fit through the proteins or lipids so the cell membrane folds over the particles and then pinches off. Exocytosis Energy needed. Active transport Large molecules, clumps of material such as waste or proteins. The whole membrane The membrane folds and pinches together to allow the particles to escape.

42. Cell size

43. Warm-up Please write down the dimensions of the two theaters below Theater A: capacity 200 people and 6 exits Theater B: capacity 800 people 16 exits Scenario: you go to the theater or to the movies. You take the prime center seats to view the movie. It is a full house (all seats filled).

44. Warm-up Theater A: capacity 200 people and 6 exits Theater B: capacity 800 people 16 exits Unfortunately, there is a fire and the theater needs to be evacuated. Which theater would you rather be in, if you were to find yourself in this unfortunate incident? Discuss as group and be prepared to report out. Explain you answer in your journal.

45. Summarize why ‘large surface area to volume ratios’ are an advantage for cells:

46. Surface-area represents ______________ of cell, while volume represent contents of a cell. The membrane allow material to enter and exit (provide passage ways). Therefore, it is an advantage to have lots of ____________ in relation to the cell volume (or a large SA/V ratio) so that material can exit and enter _________, consequently ____________ cells are quicker at getting things in and out. Just like small theater analogy.