1. Drosophila melanogaster

2. 8 Chromosomes Life Cycle Temp-dependent Stages Egg+larva--8 days
Pupa--8 days
Adult--up to 8 weeks

4. Overview of Lab Set up vials 10 mL medium + 10 mL water
5-8 grains yeast
Netting
Label: initials, date, cross. #1,2,3

5. Form Hypothesis Obtain parent phenotype. Predict:
Parents are true-breeding.
Predict:
Genotype and phenotype of F1
Genotype and phenotype of F2

6. Set up Culture Vials Anesthetize F1 vial
Sort males and females separately; 1/3 into each vial
Record # of flies put into each vial (male and female), as well as phenotypes of flies

7. Remove F1 Anesthetize and remove F1 flies once F2 pupae have darkened
About a week after pupae first appeared

8. Counting Flies
Anesthetize and count F2 flies for 2 weeks after they first appear
Record sex and genotype of each fly
Release/dispose of flies after counting

9. Statistically Analyze Data
Perform Chi-Squared test on F2 data
Accept or reject hypothesis