2. Latex resin extract, typical of Peru and Amazzonia Obtained through cortex incision of the Croton Lechleri tree Rich in Taspine, Pro-anthocyanidins, Lignams Documented cicatrizing, anti-inflammatory, anti-bacterial action..also known as Sangre de Grado or Sangre de drago CROTON LECHLERI EXTRACT

3. ACTIVE COMPONENTS CIKADERM TASPINE PROANTHOCYANIDINS LIGNAMS

4. Alkaloid molecule responsible of cicatrizing action Determines a Chemiotaxis call of fibroblasts in damaged skin tissue (..with increase of 56% fibroblast methabolism) Fibroblasts called in ‘’in loco’’ provide elastic and mechanical resistance to the dermis, by the production of new Elastic and Collagen fibers (Increased level of Collagen I, III, IV, Fibronectin, Integrin β-4) CROTON LECHLERI EXTRACT 1 - TASPINE

5. Phytoestrogen structure 3', 4-0-dimethyl-cedrusine is able to preserve the integrity of the originary tissue subjected to the damage Concomitant anti-bacterial action, CROTON LECHLERI EXTRACT 2 - LIGNAMS

6. Able to reduce inflammatory events on the skin Prevention of damage to dermis component structures Anti-oxidant activity Responsable of the speed of the scar tissue formation, through the induction of seric protein physical precipitation, CROTON LECHLERI EXTRACT 3 – PROANTHOCYANIDINS

7. IMPROVEMENT OF STRATIFICATION OF NEW EPIDERMIS INCREASE OF COLLAGEN IV EXPRESSION INCREASE OF β4 INTEGRINS EXPRESSION INCREASE FIBRONECTINE INCREASE COLLAGEN I and COLLAGEN III EXPRESSION ANTI-OXIDANT ACTIVITY INCREASE OF 56 % FIBROBLASTS ACTIVITY.. SIGNIFICANT CICATRIZING AND SKIN REJUVENATING ACTION! CROTON LECHLERI EXTRACT BIOCHEMICAL INDUCED effects (recap)

8. Taspine is the Cicatrizant Principle in Sangre de Grado Extracted from Croton lechleri. Planta Medica 55 (1989) Isolation of a dihydrobenzofuran lignan from South American dragon’s blood (Croton SPP.) As an inhibitor of cell proliferation. Journal of Natural Products Vol.56, No.6, pp. 899-906, June 1993 In vivo wound healing activity of Dragon’s Blood (Croton SPP.), a traditional South American drug, and its constituents. Phytomedicine vol.2 (1), pp. 17-22, 1995 Studies on the Anti-Tumour, Anti-Bacterial, and Wound-Healing Properties of Dragon’s Blood. Planta Medica 60 (1994) Review of Sangre de Drago (Croton lechleri)-A South American Tree Sap in the Treatment of Diarrhoea, Inflammation, Insect Bites, Viral Infections, and Wounds: Traditional Uses to Clinical Research. The Journal of Alternative and Complementary Medicine Vol.9, No. 6, 2003, pp. 877-896 Inhibition of Neurogenic Inflammation by the Amazonian Herbal Medicine Sangre de Grado. The Journal of Investigative Dermatology ( 2001)51-56 CROTON LECHLERI EXTRACT VERY WELL KNOWN COMPONENTS

9. PROANTHOCY ANIDINS Alkaloid molecule responsible of cicatrizing action Determines a Chemiotaxis call of fibroblasts in damaged skin tissue Fibroblasts called in ‘’in loco’’ provide elastic and mechanical resistance to the dermis, by the production of new Elastic and Collagen fibers

10. Significant DECREASE of stretch-marks TEXTURE 10 subjects: male and female (with scars or stretch marks) between 18-70 years Cream with Cikaderm @3% CROTON LECHLERI EXTRACT TEXTURE of scar and strecth marks

11. Significant DECREASE of stretch-marks COLORATION 10 subjects: male and female (with scars or stretch marks) between 18-70 years Cream with Cikaderm @3% CROTON LECHLERI EXTRACT APPEARANCE of scar and strecth marks

12. Significant DECREASE of the ITCHING perception 10 subjects: male and female (with scars or stretch marks) between 18-70 years Cream with Cikaderm @3% CROTON LECHLERI EXTRACT ITCHING (subjective evaluation)

13. CROTON LECHLERI EXTRACT CLINICAL CASE

14. Skin stratification Beta-4 Integrin levels Collagen I levels Croton Lechleri extract Control CROTON LECHLERI EXTRACT PHOTO EVALUATION

15. Collagen IV levels Fibronectine levels Collagen III levels ControlCroton Lechleri extract Control Croton Lechleri extract CROTON LECHLERI EXTRACT PHOTO EVALUATION

16. normalizes the aesthetic appearance of scars and stretch marks by enhancing the functional parameters of the skin CROTON LECHLERI EXTRACT CIKADERM

17. Has a eutrophic and regenerative effect Increases skin tone and elasticity Stimulates faster regeneration of damaged tissue Improves scars appearance Has a normalizing effect on scars both aesthetically and physiologically Reduces the sensation of tight skin typical of recently formed scars CROTON LECHLERI EXTRACT CIKADERM

18. STRETCH-MARK FORMATION PREVENTION Healing ‘’PINK-RED’’ STRETCH-MARKS PHYTOTERAPEUTIC CICATRIZING products NO SIDE EFFECTS known in literature SEVERAL TARGETS (Pregnancy, Body Building, fast loss of weight based stretch-mark healing)..GREEN PRODUCT CROTON LECHLERI EXTRACT APPLICATIONS & PLUS

19. Thank you Via Pastore 1 - 25082 Botticino Sera (Brescia) Italy Tel. +39 0302693532 - Fax. +39 0302193581 www.kalichem.it kalichem@kalichem.it