1. A Closer Look at Cell Membranes
Chapter 5 Part 1

2. Impacts, Issues: One Bad Transporter and Cystic Fibrosis
Transporter proteins regulate the movement of substances in and out of cells; failure of one of these proteins causes cystic fibrosis

3. 5.1 Organization of Cell Membranes
The basic structure of all cell membranes is the lipid bilayer with many embedded proteins
A membrane is a continuous, selectively permeable barrier

4. Revisiting the Lipid Bilayer
Phospholipid molecules in the plasma membrane have two parts
Hydrophilic heads interact with water molecules
Hydrophobic tails interact with each other, forming a barrier to hydrophilic molecules

5. Cell Membrane Organization

6. a phosphatidylcholine
Figure 5.2
Cell membrane organization. (a) Phosphatidylcholine, the most common phospholipid component of animal cell membranes. (b) Cholesterol, the main steroid component of animal cell membranes. Phytosterols are its equivalent in plant cell membranes. (c) Spontaneous organization of phospholipids into two layers (a lipid bilayer). When mixed with water, phospholipids aggregate into a bilayer, with their hydrophobic tails sandwiched between their hydrophilic heads.
a phosphatidylcholine
Fig. 5-2a, p. 78

7. hydrophilic (polar) parts
hydrophobic (nonpolar) parts
Figure 5.2
Cell membrane organization. (a) Phosphatidylcholine, the most common phospholipid component of animal cell membranes. (b) Cholesterol, the main steroid component of animal cell membranes. Phytosterols are its equivalent in plant cell membranes. (c) Spontaneous organization of phospholipids into two layers (a lipid bilayer). When mixed with water, phospholipids aggregate into a bilayer, with their hydrophobic tails sandwiched between their hydrophilic heads.
b cholesterol
Fig. 5-2b, p. 78

8. The Fluid Mosaic Model Fluid mosaic model
Describes the organization of cell membranes
Phospholipids drift and move like a fluid
The bilayer is a mosaic mixture of phospholipids, steroids, proteins, and other molecules

9. Fluid Mosaic Model

10. one layer of lipids one layer of lipids fluid c lipid bilayer fluid
Figure 5.2
Cell membrane organization. (a) Phosphatidylcholine, the most common phospholipid component of animal cell membranes. (b) Cholesterol, the main steroid component of animal cell membranes. Phytosterols are its equivalent in plant cell membranes. (c) Spontaneous organization of phospholipids into two layers (a lipid bilayer). When mixed with water, phospholipids aggregate into a bilayer, with their hydrophobic tails sandwiched between their hydrophilic heads.
c lipid bilayer
fluid
Fig. 5-2c, p. 78

11. Variations on the Model
Differences in membrane composition
Different kinds and numbers of carbohydrates attached to membrane proteins
Different kinds of phospholipids
Differences in fluidity
Some proteins are attached to the cytoskeleton; others drift around
Archaeans have more rigid membranes than bacteria or eukaryotes

12. Two Studies of Membrane Structure

13. Figure 5.4
Two studies of membrane structure, an observation and an experiment.
Fig. 5-4a, p. 79

14. Figure 5.4
Two studies of membrane structure, an observation and an experiment.
A Researchers first froze a cell membrane, then they split apart the two layers of its lipid bilayer. Microscopic analysis revealed many proteins embedded within the lipid bilayer.
Fig. 5-4a, p. 79

15. Figure 5.4
Two studies of membrane structure, an observation and an experiment.
Fig. 5-4b, p. 79

16. fusion into hybrid cell proteins from both cells
human cell
mouse cell +
fusion into hybrid cell
proteins from both cells
in fused membrane
Figure 5.4
Two studies of membrane structure, an observation and an experiment.
B Cells of two species were fused into a hybrid cell. In less than one hour, most of the plasma membrane proteins from both species had drifted through the hybrid cell’s lipid bilayer and intermingled.
Fig. 5-4b, p. 79

17. 5.2 Membrane Proteins
Cell membrane function begins with the many proteins associated with the lipid bilayer

18. Membrane Proteins
Each type of protein in a membrane has a special function
Adhesion proteins
Recognition proteins
Receptor proteins
Enzymes
Transport proteins (active and passive)

19. Common Types of Membrane Proteins

20. Types of Membrane Proteins

21. Figure 5.4
Two studies of membrane structure, an observation and an experiment.
A Researchers first froze a cell membrane, then they split apart the two layers of its lipid bilayer. Microscopic analysis revealed many proteins embedded within the lipid bilayer.
Fig. 5-4a, p. 79

22. fusion into hybrid cell proteins from both cells
human cell
mouse cell +
fusion into hybrid cell
proteins from both cells
in fused membrane
Figure 5.4
Two studies of membrane structure, an observation and an experiment.
B Cells of two species were fused into a hybrid cell. In less than one hour, most of the plasma membrane proteins from both species had drifted through the hybrid cell’s lipid bilayer and intermingled.
Fig. 5-4b, p. 79

23. Animation: Fusing membranes

24.  Adhesion Protein Enzyme Receptor Protein Recognition Protein
Passive Transporter
Active Transporter
Figure 5.5
Major categories of membrane proteins, with descriptions and examples. You will see the icons above some of the descriptions again in this book.
Transporters span all cell membranes. The other proteins shown are components of plasma membranes. Organelle membranes also incorporate additional kinds of proteins.
Stepped Art
Fig. 5-5, pp

25. Animation: Cell membranes

26. 5.1-5.2 Key Concepts: Membrane Structure and Function
Cell membranes have a lipid bilayer that is a boundary between the outside environment and the cell interior
Diverse proteins embedded in the bilayer or positioned at one of its surfaces carry out most membrane functions

27. 5.3 Diffusion, Membranes, and Metabolism
Ions and molecules tend to move from one region to another, in response to gradients

28. Membrane Permeability
Selective permeability
The ability of a cell membrane to control which substances and how much of them enter or leave the cell
Allows the cell to maintain a difference between its internal environment and extracellular fluid
Supplies the cell with nutrients, removes wastes, and maintains volume and pH

29. The Selectively Permeable Nature of Cell Membranes

30. and carbon dioxide), small nonpolar molecules, and
A Gases (such as oxygen
and carbon dioxide), small
nonpolar molecules, and
water cross a bilayer freely.
B Other solutes
(molecules and ions)
cannot cross a lipid
bilayer on their own.
Lipid
Bilayer
Figure 5.6
The selectively permeable nature of cell membranes. Small, nonpolar molecules, gases, and water molecules freely cross the lipid bilayer. Polar molecules and ions cross with the help of proteins that span the bilayer.
Fig. 5-6, p. 82

31. and carbon dioxide), small nonpolar molecules, and
A Gases (such as oxygen
and carbon dioxide), small
nonpolar molecules, and
water cross a bilayer freely.
B Other solutes
(molecules and ions)
cannot cross a lipid
bilayer on their own.
Lipid
Bilayer
Figure 5.6
The selectively permeable nature of cell membranes. Small, nonpolar molecules, gases, and water molecules freely cross the lipid bilayer. Polar molecules and ions cross with the help of proteins that span the bilayer.
Stepped Art
Fig. 5-6, p. 82

32. Animation: Selective permeability

33. Concentration Gradients
The number of molecules (or ions) of substance per unit volume of fluid
Concentration gradient
The difference in concentration between two adjacent regions
Molecules move from a region of higher concentration to one of lower concentration

34. Diffusion
Diffusion
The net movement of molecules down a concentration gradient
Moves substances into, through, and out of cells
A substance diffuses in a direction set by its own concentration gradient, not by the gradients of other solutes around it

35. Examples of Diffusion

36. dye
A Dye is dropped into a bowl of water. The dye molecules diffuse until they are evenly dispersed among the water molecules.
Figure 5.7
Examples of diffusion.
B Red dye and yellow dye are added to a bowl of water. Each substance moves according to its own concentration gradient until all are evenly dispersed.
Fig. 5-7, p. 82

37. Figure 5.7
Examples of diffusion.
Fig. 5-7a, p. 82

38. dye
Figure 5.7
Examples of diffusion.
A Dye is dropped into a bowl of water. The dye molecules diffuse until they are evenly dispersed among the water molecules.
Fig. 5-7a, p. 82

39. Figure 5.7
Examples of diffusion.
Fig. 5-7b, p. 82

40. Figure 5.7
Examples of diffusion.
B Red dye and yellow dye are added to a bowl of water. Each substance moves according to its own concentration gradient until all are evenly dispersed.
Fig. 5-7b, p. 82

41. The Rate of Diffusion Rate of diffusion depends on five factors Size
Temperature
Steepness of the concentration gradient
Charge
Pressure

42. How Substances Cross Membranes
Gases and nonpolar molecules diffuse freely across a lipid bilayer
Ions and large polar molecules require other mechanisms to cross the cell membrane
Passive transport
Active transport
Endocytosis and exocytosis

43. Membrane-Crossing Mechanisms

44. Figure 5.8
Overview of membrane-crossing mechanisms.
Fig. 5-8 (a-c), p. 83

45. A Diffusion A substance simply dif- fuses across lipid bilayer.
Extracellular Fluid
Cytoplasm
Figure 5.8
Overview of membrane-crossing mechanisms.
A Diffusion A substance simply dif- fuses across lipid bilayer.
B Passive Transport
A solute moves across bilayer through interior of passive transporter; movement is driven by concentration gradient.
C Active Transport Active transporter uses energy (often, ATP) to pump a solute through bilayer against its concentration gradient.
Fig. 5-8 (a-c), p. 83

46. Figure 5.8
Overview of membrane-crossing mechanisms.
Fig. 5-8 (d-e), p. 83

47. D Endocytosis Vesicle movement brings substances in bulk into cell.
Figure 5.8
Overview of membrane-crossing mechanisms.
E Exocytosis Vesicle movement ejects substances in bulk from cell.
Fig. 5-8 (d-e), p. 83

48. 5.4 Passive and Active Transport
Many types of molecules and ions diffuse across a lipid bilayer only with the help of a specific transport protein

49. Passive Transport Passive transport (facilitated diffusion)
Requires no energy input
A passive transport protein allows a specific solute (such as glucose) to follow its concentration gradient across a membrane
A gated passive transporter changes shape when a specific molecule binds to it

50. Passive Transport

51. Figure 5.9
Passive transport. This model shows one of the glucose transporters that span the plasma membrane. Glucose crosses in both directions. The net movement of this solute is to the side of the membrane where it is less concentrated.
Fig. 5-9a, p. 84

52. Extracellular Fluid glucose Cytoplasm lipid bilayer
Figure 5.9
Passive transport. This model shows one of the glucose transporters that span the plasma membrane. Glucose crosses in both directions. The net movement of this solute is to the side of the membrane where it is less concentrated.
A A glucose molecule (here, in extracellular fluid) binds to a transport protein embedded in the lipid bilayer.
Fig. 5-9a, p. 84

53. Figure 5.9
Passive transport. This model shows one of the glucose transporters that span the plasma membrane. Glucose crosses in both directions. The net movement of this solute is to the side of the membrane where it is less concentrated.
Fig. 5-9b, p. 84

54. B Binding causes the protein to change shape.
Figure 5.9
Passive transport. This model shows one of the glucose transporters that span the plasma membrane. Glucose crosses in both directions. The net movement of this solute is to the side of the membrane where it is less concentrated.
B Binding causes the protein to change shape.
Fig. 5-9b, p. 84

55. Figure 5.9
Passive transport. This model shows one of the glucose transporters that span the plasma membrane. Glucose crosses in both directions. The net movement of this solute is to the side of the membrane where it is less concentrated.
Fig. 5-9c, p. 84

56. Figure 5.9
Passive transport. This model shows one of the glucose transporters that span the plasma membrane. Glucose crosses in both directions. The net movement of this solute is to the side of the membrane where it is less concentrated.
C The glucose molecule detaches from the transport protein on the other side of the membrane (here, in the cytoplasm), and the protein resumes its original shape.
Fig. 5-9c, p. 84

57. Animation: Passive transport I

58. Active Transport Active transport Calcium pumps
Requires energy input (usually ATP)
Moves a solute against its concentration gradient, to the concentrated side of the membrane
Calcium pumps
Active transporters move calcium ions across muscle cell membranes into the sarcoplasmic reticulum

59. Active Transport: Calcium Pump

60. Figure 5.10
Active transport. This model shows a calcium transporter. After two calcium ions bind to the transporter, ATP transfers a phosphate group to it, thus providing energy that drives the movement of calcium against its concentration gradient across the cell membrane.
Fig. 5-10a, p. 85

61. Sarcoplasmic Reticulum
calcium
Sarcoplasmic Reticulum
Cytoplasm
Figure 5.10
Active transport. This model shows a calcium transporter. After two calcium ions bind to the transporter, ATP transfers a phosphate group to it, thus providing energy that drives the movement of calcium against its concentration gradient across the cell membrane. A
Calcium ions bind to a calcium transporter (calcium pump).
Fig. 5-10a, p. 85

62. Figure 5.10
Active transport. This model shows a calcium transporter. After two calcium ions bind to the transporter, ATP transfers a phosphate group to it, thus providing energy that drives the movement of calcium against its concentration gradient across the cell membrane.
Fig. 5-10b, p. 85

63. energy
ATP
Figure 5.10
Active transport. This model shows a calcium transporter. After two calcium ions bind to the transporter, ATP transfers a phosphate group to it, thus providing energy that drives the movement of calcium against its concentration gradient across the cell membrane.
B A phosphate group is transferred from ATP to the pump. The pump changes shape so that it ejects the calcium ions to the opposite side of the membrane, and then resumes its original shape.
Fig. 5-10b, p. 85

64. Cotransport Cotransporter
An active transport protein that moves two substances across a membrane at the same time
Example: The sodium-potassium pump moves Na+ out of the cell and K+ into the cell

65. Cotransport: Sodium-Potassium Pump

66. Figure Cotransport. This model shows how a sodium–potassium pump transports sodium ions (Na+, red ) from the cytoplasm to the extracellular fluid, and potassium ions (K+, purple) in the other direction across the plasma membrane. A phosphate group transfer from ATP provides energy for the transport.
Figure 5.11
Cotransport. This model shows how a sodium–potassium pump transports sodium ions (Na+, red) from the cytoplasm to the extracellular fluid, and potassium ions (K+, purple) in the other direction across the plasma membrane. A phosphate group transfer from ATP provides energy for the transport.
Fig. 5-11, p. 85

67. Figure 5.11
Cotransport. This model shows how a sodium–potassium pump transports sodium ions (Na+, red) from the cytoplasm to the extracellular fluid, and potassium ions (K+, purple) in the other direction across the plasma membrane. A phosphate group transfer from ATP provides energy for the transport.
Stepped Art
Fig. 5-11, p. 85

68. 5.3-5.4 Key Concepts: Diffusion and Membrane Transport
Gradients drive the directional movements of substances across membranes
Transport proteins work with or against gradients to maintain water and solute concentrations

69. Animation: Lipid bilayer organization