1. 1 Introduction to Neurobiology Lecture 10: The inferior olive
The inferior olivary nucleus is the only source of cerebellar climbing fibers

2. The Inferior Olive nucleus2
The Inferior Olive nucleus

3. Olivary neurons (Ramon Y Cajal)3

4. HRP filled olivary neuron
Intracellular labeling of olivary neuron 4

5. Action potential in olivary neuron5

6. Action potentials in olivary neuron6

7. The effect of TTX on action potentials in olivary neuron7

8. 8
The effect of Mn2+ and Cd2+ on action potentials in olivary neuron

9. Two types of action potentials in olivary neuron9
-60 mV
-55 mV
-60 mV
-68 mV
-60 mV
15 mV
15 mV
1 nA
20 msec

10. The Na+ and Ca2+ components of the two types of action potentials10

11. Independent activation of the action potentials11
TTX

12. Refractory period of the action potentials12
Refractory period of the action potentials

13. Refractory period of the action potentials13
Refractory period of the action potentials
10 mV

14. The Inactivation curve of the low threshold action potential14

15. The effect of Harmaline on Olivary neuron.15
The effect of Harmaline on Olivary neuron.

16. The effect of harmaline on the Inactivation curve.16
The effect of harmaline on the Inactivation curve.
control
harmaline
Resting potential
control
harmaline

17. The effect of Octanol on Olivary neuron.17
The effect of Octanol on Olivary neuron.

18. Possible mechanism of rhythmic activity18
Possible mechanism of rhythmic activity
Ionic currents in Olivary neurons:
Na current – typical H&H current
Dendritic Ca current – high threshold
non-inactivating
Ca dependent, K current
Somatic Ca current – low threshold
inactivating

19. Electrical coupling between olivary neuron19
Electrical coupling between olivary neuron
Cell 1
Cell 2
20 mV
Cell 1
Cell 2
5 mV
200 ms
Cell 1
Cell 2
Cell 1
Cell 2
2 mV
100 ms
20 mV
200 ms
10 mV
10 mV 5

20. Dye coupling between olivary neuron20
Dye coupling between olivary neuron

21. The olivary glumerulus21
The olivary glumerulus

22. 22
Olivary Gap junction