1. Group members:Benjun Liu Junjie Yang Runmin Zhang Yuxiang Qin

2.  Introduction  Experimentation  Outlook Contents

3.  Introduction  Experimentation  Outlook Contents

4. Why ? How?

6. Ag NPs CTAB “glues” MUA “switch” Ag Dimers

7. CTAB and MUA’s roles in synthesizing Ag Dimers

8.  Introduction  Experimentation  Outlook Contents

9. Preparation of Ag NPs Glass slide’s Modification with APS Self-Assembly of Ag Dimer Experiment Section

10. PVP@EG 10g PVP(MW=10000)@75mL EG +Ag 400mg AgNO 3 added with continuous stirring Heat room temperature to 120℃ 1℃/min then 1h at 120℃

12. Small peak at 600nm ——Overheated ？

13. PVP@EG 10g PVP(MW=10000)@75mL EG +Ag 400mg AgNO 3 added with continuous stirring Heat 30min at 120℃

15. Clean glass slides Immerse the slides into APS solutions for 12h Rinse with extensive water

16. Ag NPs CTAB 0.1mM×0.2mL 15min MCA 0.2mM×1mL Self- assemble

17. Ag NPs CTAB 0.1mM×0.2mL 15min MPA 0.2mM×1mL Self- assemble

18. Absorption Spectrum

19. UnannealedAnnealed

20. UnannealedAnnealed

21. Ag NPsAg Dimers

24.  Introduction  Experimentation  Outlook Contents

25. Without bringing in any external label, fluorescence enhancements with Ag Dimers could detect DNA/RNA sensitively Direct and non-damage detection of DNA/RNA provide an important new tool for management of patients with other cancers Fast Detection of Circulating Tumor Cells

26. Lakowicz J R, Shen B, Gryczysnki Z, et al. Biochem. Biophys. Res. Commun., 2001, 286 : 875 —879 DNA/RNA signals: weak, short life Ag Dimers: Enhancing fluorescence signals

27. Traditional Methods ： Cell Culturing Low Specificity Low Sensitivity Time Consuming Surface-Enhanced Raman Spectroscopy Specific Sensitive Convenient

28.  Could probably improve the intensity with Ag dimers Xu Wang, Ximei Qian, Jonathan J. Beitler, et al. ， Cancer Res 2011;71:1526-1532

29.  Thanks for your listening!