1. Mass production and industrialization of potato via biotechnology using plant tissue culture system
국제기구- 지구환경보전실천기구
INGO-GECPO Project
Pumasi Korea Co. ltd.

2. Mass production and industrialization of potato via biotechnology using plant tissue culture system
Biotechnology Virus-free stock Mass production Potato

3. Process of plant cell and tissue culture
植物体
Shoot induction
Root Induction
Plant
Seed
(Plant Nusery)
Somatic embryo
plantlets
Cell culture
植物体
proliferation
Callus
幼植物体
大量生産
Tissue
Solid culture
liquid culture
不定根の
大量生産
(Mass production)
Root

4. Habituation of plantlets derived from
somatic embryos
Since 1998 국내최초, 세계 2번째 배양체 건강식품

5. world`s four major crops
Harvest of
world`s four major crops
(2009, FAO STAT)
Harvest
(1,000t)
(㎏/㏊)
Rice
575,430
3,899
Wheat
572,667
2,683
Corn
604,407
4,385
Potato
307,440
16,130

6. Harvest of potato Cultivation area Country (tone/㏊) (1,000㏊)
(2009, FAO STAT)
Country
(consumption)
kg /person /yr)
Harvest yield(1,000t)
Cultivation area
(1,000㏊)
(tone/㏊)
(seed supply%)
China (32.5)
73,282
5,083
(남한국토면적의 1/2)
14 (10%)
Russia (133.5)
31,134
2,182
India (17.9)
3,439
1,828
19 (10%)
USA (53.4)
19,569
   419
47 (90%)
Germany (69.5)
11,617
   264
44 (90%)
Nederland (92.0)
7,181
   155
46 (90%)
North KOR (2003)
1,884
   198
  10
Korea (13.5)
  591
    21
28 (40%)

7. Process of tissue culture and seed production in potato
Old seed production system ( after 1980)
Seed potato cutting
From shoot tip culture
Previous culture system (after 2000)
Seed potato cutting
Mass production using Bioreactor system
Without cutting
Foundation
seed
Breeder seed
Farm

8. Right: virus infection zone
Potato cultivation from virus-free stock
Left: virus-free zone
Right: virus infection zone

9. Problem and resolution of tissue culture
previos resolution
New resolution
1. Sucrose addition
New
bio
tech.
Without su, High light
Indu
strial
ization
- Contamination factor
2. Close of culture bottle
Air circulation
- Growth inhibition, gas
3. Solid culture
Liquid culture
- Small volume, High-cost
Plant factory
Previous method

10. 1-2. Tissue culture & shoot proliferation in bioreactor
Potato stem culture (1Kg)
Light condition
bioreactor
reproliferation
Mass stem culture (11Kg)

11. Mass production of potato tuber using bioreactor
light culture
Plants
Potato tuber
Dark culture

12. Green house Cultivation of plantlets and tuber of potato

13. Inside view of Plant factory for potato production
Plant nursery Plant factory
Inside view of nursery

15. Breeder seed production from Potato micro tuber

16. Process of virus-free potato productoion Cultivation in green house
Reproliferation
shoot
shoot
light
bioreactor
dark
Cutting Plantlets
Tuber formatiom
Rooting
Cultivation in green house
Breeder seed
Foundation seed
기본종
씨감자
Foundation seed
원원종
씨감자
High density
cultivation
cultivation
Edible potatoes
High yield

17. 7. Soil Cultivation of seed potato