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Diffusion and Osmosis.

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Presentation on theme: "Diffusion and Osmosis."— Presentation transcript:

1 Diffusion and Osmosis

2 Outline Learn the concepts of: Explore diffusion in a colloid
Diffusion, osmosis, semi-permeable membrane, isotonic, hypertonic, & hypotonic Explore diffusion in a colloid Relationship between diffusion & size Explore diffusion & osmosis in a dialysis bag Dialysis bag experiment Semi-permeable membrane Water, glucose, starch Plant/animal cells exposure to water Hypertonic Env. Hypotonic Env. Isotonic Env. 2 3 1 4

3 Diffusion Solute molecules moving from an area of high concentration to an area of low concentration Random motion drives diffusion Movement is based on kinetic energy (speed), charge, and mass of molecules Equilibrium is reached when there is an even distribution of solute molecules (water) 2 3 1 4

4 Osmosis Diffusion of water through a semi-permeable membrane
Semi-permeable: permeable to solvents (WATER), but not to large molecules High [water] to low [water] Dissolved molecules (i.e. glucose, starch) are called solutes REMEMBER: Water = solvent Glucose, Starch = solutes

5 Effect of Water on Cells
Hypertonic Environment High [solute], low [water] Hypotonic Environment High [water], low [solute] Isotonic Environment [water] = [solute] Hypertonic Hypotonic Isotonic Part 3 pg. 85

6 Osmosis in Living Cells
Cellulose in cell wall

7 Transportation of Molecules
Passive Transport Movement of molecules across a semi-permeable membrane no energy required Active Transport Movement of molecules across a semi-permeable membrane against a concentration gradient with a protein ENERGY – ATP Facilitated Diffusion Movement of molecules across a semi-permeable membrane with a protein

8 Effect of Water on RBC

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12 Dialysis Bag Experiment
Iodine Glucose Starch Dialysis bag T0 T80 Weigh Dialysis Bag Semi-permeable membrane Water, glucose, & starch What passes through the dialysis bag? Glucose (Benedict’s Test) Starch (Iodine Solution) Time Course Experiment Every 10 minutes, measure the amount of glucose & starch present in the beaker Part 2 pg. 83

13 Dialysis Bag Experiment - Results
Lab Manual, page 84

14 Dialysis Bag Experiment - Controls
Negative control for dialysis bag experiment (performed by TA) Instead of using dialysis tubing, I will be using a plastic bag. I will determine the weight, Benedict‘s result, & iodine results at time 0 and 80 minutes Biochemical test controls (in pairs) set up test control of iodine test w/ glucose and starch set up test control of Benedict‘s test w/ glucose and starch Iodine Benedict’s Glucose ? Starch

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