1. Infections Due to Malassezia Downloded from www.pharmacy123.blogfa.com

2. Definition Various species of Malassezia cause both opportunistic, superficial infections and occasionally systemic infections Common superficial infections include: –Pityriasis versicolor –Seborrheic dermatitis –Atopic dermatitis –Folliculitis –Dandruff Downloded from www.pharmacy123.blogfa.com

3. Case Report 1 Fan et al., Arch. Dermatol. (2006) 142: 1181- 1184. In January 2004, a 49 year-old female developed an asymptomatic facial papule Self-treated with herbs, but became larger, erosive, and produced an exudate Downloded from www.pharmacy123.blogfa.com

4. Case Report 1 (cont.) In March 2004, a similar nasal lesion appeared Patient presented to clinic in April 2004 –No history of trauma –Owned a pet dog for 9 months –No lymphadenopathy Downloded from www.pharmacy123.blogfa.com

5. Source: Fan et al., Arch. Dermatol. 142 (2006) 1181-1184 Downloded from www.pharmacy123.blogfa.com

6. Case Report 1 (cont.) Biopsy of lesion –Gram stain revealed numerous budding yeast cells –Pronounced inflammatory reaction including microabscesses of follicules and numerous lymphocytes and histiocytes –PAS staining documented round-to-ovoid cells/spores in necrotic areas as well as in dermis Downloded from www.pharmacy123.blogfa.com

7. Source: Fan et al., Arch. Dermatol. 142 (2006) 1181-1184 Downloded from www.pharmacy123.blogfa.com

8. Case Report 1 (cont.) Culture/Laboratory Work –Skin scrappings from both patient and dog grew yeast-like cells on Sabouraud Dextrose agar with or without olive oil supplement –Scanning electron micrographs revealed morphology consistent with Malassezia pachydermatis –Patient had no other underlying disease or immunosuppression Downloded from www.pharmacy123.blogfa.com

9. Case Report 1 (cont.) Treatment –Initially treated with antituberculosis agents because of slow culture results –After positive fungus culture results, patient was treated with itraconazole and potassium iodide –Lesion stopped growing but was still positive for fungus –Therapy changed to fluconazole with cryotherapy to remove lesion –Some hypopigmented scarring remained, but patient was free of infection after 15 months Downloded from www.pharmacy123.blogfa.com

10. Source: Fan et al., Arch. Dermatol. 142 (2006) 1181-1184 Downloded from www.pharmacy123.blogfa.com

11. Case Report 2 Rosales et al., Ped. Develop. Pathol. (2004) 7: 86-90. Infant born after 23 weeks of gestation –Chronic lung disease –Necrotizing enterocolitis –Intraventricular hemorrahge –At 24 days post birth, developed hypotension Treated empirically with amphotericin B Hepatic lesion noted Downloded from www.pharmacy123.blogfa.com

12. Case Report 2 (cont.) –Blood cultures were positive for Malassezia furfur on day 11 of treatment (day 35 of life) –Central line catheter was also shown to be positive for M. furfur –Removal of catheter resulted in negative fungus cultures for 2 weeks of amphoterin B therapy Downloded from www.pharmacy123.blogfa.com

13. Case Report 2 (cont.) –Day 50 of life Patient’s condition worsened due to intestinal perforation Surgery improved condition and was being given intravenous hyperalimentation infusions of lipids via a scalp catheter Downloded from www.pharmacy123.blogfa.com

14. Case Report 2 (cont.) –Day 83 of life Patient’s condition worsened again and seizure occurred Spinal fluid examination revealed fungal forms consistent with M. furfur Catheter and blood cultures were positive for M. furfur Death occurred on day 86 Downloded from www.pharmacy123.blogfa.com

15. Source: Rosalles et al., Ped.Devel. Pathol. 7 (2004) 86-90 Downloded from www.pharmacy123.blogfa.com

16. Case Report 2 (cont.) Autopsy findings –Inflammatory reactions of meninges consistent with meningitis –Histopathological examination (silver stained sections) revealed meninges contained yeast cells with morphologies consistent with M. furfur –No such observations were noted for any other organs in the body Downloded from www.pharmacy123.blogfa.com

17. Source: Rosalles et al., Ped.Devel. Pathol. 7 (2004) 86-90 Downloded from www.pharmacy123.blogfa.com

18. Pityriasis Versicolor Synonym: tinea versicolor, among others Presentation: –Chronic, benign skin disorder –Asymptomatic –Characterized by scaly patches of varible color (pink, white, or brown) of the upper trunk –Worldwide in distribution Downloded from www.pharmacy123.blogfa.com

19. Pityriasis Versicolor (cont.) Etiological Agents: –Various species of Malassezia: M. furfur M. globosa M. sympodialis M. sloofiae M. restricta Downloded from www.pharmacy123.blogfa.com

20. Pityriasis Versicolor (cont.) –There are other species of Malassezia which may or may not be involved in pityriasis versicolor M. obtusa M. pachydermatis - common pathogen of dogs –Malassezia is a basidiomycetous yeast, but the telomorph has yet to be described –Different species differentiated based upon: Physiological parameters, including use of complex lipid sources Genetic-based differences Downloded from www.pharmacy123.blogfa.com

21. Pityriasis Versicolor (cont.) Epidemiology: –Typically an infection of children and young adults –Associated with hormonal changes and increased sebum production –Favored by high temperature and humidity, particularly tropic areas Downloded from www.pharmacy123.blogfa.com

22. Pityriasis Versicolor (cont.) Clinical manifestations –Multiple macules and/or patches varying in appearance Hypopigmented Hyperpigmented Erythematous –Commonly affected areas include back, chest, abdomen, neck, and upper limbs –Children often acquire facial macular lesions Downloded from www.pharmacy123.blogfa.com

23. Pityriasis versicolor. Source: www.doctorfungus.com Downloded from www.pharmacy123.blogfa.com

24. Lesions of pityriasis versicolor. Source: www.doctorfungus.com Downloded from www.pharmacy123.blogfa.com

25. Pityriasis Versicolor (cont.) Diagnosis –Typically, KOH preps of lesions that show yeast and pseudohyphal elements (“spaghetti and meat balls”) –Can confirm the diagnosis by using a Wood's lamp to show yellow to yellow-green fluorescence of active lesions. Downloded from www.pharmacy123.blogfa.com

26. “Spaghetti and Meat Balls” - Malassezia furfur (PAS stain). Source: www.doctorfungus.com Downloded from www.pharmacy123.blogfa.com

27. Pityriasis Versicolor (cont.) Treatment is via use of topical agents including: –Selenium sulfide shampoo –Zinc pyrithione shampoo –Ciclopirox –Terbinafine –Benzoyl peroxide Downloded from www.pharmacy123.blogfa.com

28. References Clinical Mycology. 2003. Elias J. Anaissie, Michael R. McGinnis, Michael A. Pfaller, eds. Churchill Livingstone Clinical Mycology. 2003. William E. Dismukes, Peter G. Pappas, Jack D. Sobel, eds. Oxford University Press Topley & Wilson's Microbiology & Microbial Infections, 10th ed. 2005. Vol. 3. Medical Mycology. Hodder Arnold Downloded from www.pharmacy123.blogfa.com

29. References (cont.) www.doctorfungus.com (accessed on June 3, 2007) Ashbee, H. R. 2007. Update on the genus Malassezia. Med. Mycol. 4: 287-303. Morishita, N., Sei, Y. 2006. Microreview of pityriasis versicolor and Malassezia species. Mycopathologia 6: 373-376. Downloded from www.pharmacy123.blogfa.com