1. Overview of the Inc 27-28 Experiments supported by CADMOS Alain MAILLET

2. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 2 CADMOS Ops Leads Team Ops LeadExperiments Hélène RugetDOSIS, SOLO, NEUROSPAT, EPM, CARD, PASSAGES, MARES Cécile ThevenotSOLO, DOSIS, CARD, EPM, PASSAGES Stéphanie HerrCARD, PASSAGES, NEUROSPAT, SOLO, 3D SPACE, MARES Maurice MarnatPASSAGES, NEUROSPAT, 3D SPACE François NuyttensSOLO, NEUROSPAT, MARES Nicolas SprengerDOSIS Alain MailletSOLO, 3D SPACE, CARD, DOSIS, EPM, MARES  2 new persons arriving @ CADMOS in 2011 that will enter the process Stéphanie Nicolas Maurice François Hélène Cécile

3. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 3 Increment 27-28 (Inc 29-30) CARD: Investigating Mechanisms of Heart Disease (support to DAMEC) 3D-SPACE: Mental Representation of Spatial Cues During Spaceflight SOLO: Sodium retention in microgravity PASSAGES: Scaling Body-Related Actions in Absence of Gravity IMMUNO: Neuroendocrine and immunological changes NEUROSPAT: Prefrontal brain functions and spatial cognition MARES: Commissioning part 2 SARCOLAB: Myotendinous and Neuromuscular Adaptation to long-term Spaceflight: Determinants and time courses. EPM maintenance: Video Unit Replacement BDC only: EDOS: Early Detection of Osteoporosis in Space (ground only) SPIN: Centrifugation, static tilt and orthostatic tilt tests (ground only) “Specials”: DOSIS: Dose Distribution inside ISS (support to MUSC) ICV: Integrated Cardiovascular (support to NASA-ICV for Cardiopres) VESSEL IMAGING: Vascular Imaging (support to DAMEC) Experiment Support

4. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 4 Science overview Experiment that intends to test: 1.How the cardiovascular system adapts to prolonged weightlessness and whether the arterial resistance vessels are chronically dilated in space. 2.Whether there is a mismatch between the level of sympathetic nervous activity and dilatation of these vessels. Main Hardware Pulmonary Function System (Cardiac output - DAMEC) CDL Holter Arterial BP Equipment (24h BP measurements - CADMOS) – FM Spare/41P Blood Sample Kit, ESA Urine Collection Kit (CADMOS), HRF-2 RC and MELFI Subjects (6 desired, 8 preferred) Inc 26-27: P. Nespoli (prime - #7), C. Coleman (Reserve #8 – pending urine collection feedback) Inc 28-29: S. Furukawa (Prime #9 – if CC not performed, will be the last subject) Implementation 1 in flight session per subject, preferably after 3 months on-orbit, minimal 2 months. Crew time Day 0: 10 min (CDL Holter batteries insertion) Day 1: 175 min ( EPM laptop activation for Holter configuration, PFS S/U, Holter S/U, Start of 24hr urine collection, 3 PFS measurements, Holter data saving) Day 2: 240 min (Blood collection, RC, MELFI insertion, end of urine collection, urine sampling and MELFI insertion, 2 PFS measurements, Holter data saving, stowage) Data Holter data / Urine Log stored on PCMCIA card – downlink to ground via EPM Photo / Video Video of one rebreathing session CARD

5. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 5 Science overview Stand-alone experiment : the subject is immerged in a virtual environment while free floating. Software Tasks : drawing, distance, illusion Main Hardware Laptop, head mounted display, trackball, digital tablet and pen, Ultrabay Hard Disk, PCMCIA card Subjects (Requirement after Mid term review: 8 subjects) Inc 26-27: Paolo Nespoli #7, Catherine Coleman #8 (On Going) Inc 28-29: Mike Fossum #9 (pending PN and CC results, if successful MF won’t be performed – end of experiment) Implementation 4 to 5 sessions per subject Crew time Installation: 15 min Experimental session: 30 min Stowing : 15 min +10 min for OCA transfer during the last session Data Data stored on PCMCIA card – downlink to ground via OCA then TSC/Web Mirage Photo / Video 2 Photos/session are desired for evaluation of subjects head and body position during free-floating Mental Representation of Spatial Cues During Spaceflight 3D-SPACE

6. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 6 Sodium retention in microgravity Science overview Study the impact of diet period on fluid and salt retention. Main Hardware EPM Laptop for PCBA SW update CDL Holter Arterial BP Cable for PCBA SW update PCBA: Portable Clinical Blood Analyzer MELFI: Freezer HRF2/RC: Refrigerated Centrifuge HRF1/SLAMMD: space weight-scale Consumables for Blood and Urine samples Next subjects P. Nespoli, R. Garan, D. Burbank (R), A. Kuipers Implementation 2 sessions per subject, 6 days activities /session L + 30 days < session 1 < 0 to 7 days < session 2 < R – 30 days Crew time Day 1, 2, 3, 4 & 5 : diet monitoring Day 4 & 6 : body mass measurement with SLAMMD Day 5 : blood collection and PCBA measurement Day 5 & 6 : 24hrs urine collection Data Data record in logs (diet, PCBA, urine) Data downlink to ground (SLAMMD) Blood and urine samples download to ground (frozen) SOLO

7. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 7 Scaling Body-Related Actions in Absence of Gravity Science overview Stand-alone experiment. Subject quasi free floating uses a tunnel (lighshield) to immerge into a virtual corridor and evaluate « passability » through doors. Main Hardware Laptop, lightshield, trackball, PCMCIA card Next subjects S. Kelly, P. Nespoli, C. Coleman, R. Garan, M. Fossum, S. Furukawa, (D. Burbank, A. Kuipers) Implementation 2 sessions per subject (Before FD21 and after FD120) Crew time 85 min crew time / session Data Data stored on PCMCIA card – downlink to ground via EPM Photo / Video 5 min video required PASSAGES

8. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 8 Science overview Stand alone experiment to study how human psycho-neuroendocrine and immunologic systems adapt to space environment, and how these processes are dependent on the cellular level of signal processing Main Hardware Plasma03 centrifuge MELFI freezer Next subjects Russian crew member Implementation First session during quiet period between L+4m and L+5m Second session during stressed period after R-12d Crew time 3h55 min crew time /session (24h session from 8am to 8am) Day 1:Stress Tests, Saliva Samplings, Blood Sampling, Urine Collection Day 2: End of urine collection Data Urine and blood samplings (frozen), paper Current Stress tests sheets, blood smears boxes and saliva tubes, urine and blood sampling logbooks. Photo / Video Not required Neuroendocrine and immune responses during and after long term stay at ISS IMMUNO

9. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 9 Prefrontal brain functions and spatial cognition Science overview Subject in free floating using a tunnel (lighshield) to immerge into challenging scene. EEG, EOG, EMG and ECG signals are acquired via EPM-MEEMM. Main Hardware EPM, MEEMM, laptops, lightshield, gamepad, EEG cap, consumable (gel), chest belt Next subjects Paolo Nespoli, Catherine Coleman (operator only) (No subject during Inc27-28; Next subject A. Kuipers) Implementation 2 sessions per subject (around FD15 and FD60) 3 days activities /session Crew time Day 1 : hardware setup Day 2 : electronic hardware activation, EEG cap setup, experimental session, quick stowing Day 3 : data saving, stowing Data Data stored on MEEMM and laptop Neurosciences Hard Disks Data downlink via EPM High Rate link Photo / Video 5 min video required, photo-modeling pictures of EEG cap NEUROSPAT

10. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 10 Muscle Atrophy Research and Exercises System: C/O Part 2 Science overview N/A: Commissioning Part 2 = operational test of MARES Main Hardware MARES, EPM Laptop & Ext. HD, PEMS-2, CDL ADAS (+EMG electrodes), HRF Echo, consumables, EPM Rack Next subjects A. Kuipers, D. Petit (operator only) Implementation 1 session (between FD2 and FD10) 2 days activities /session Crew time Day 1 : MARES deployment + activation + Battery charge + PEMS test + Ultrasound activity Day 2 : Subject equipment (PEMS-2 + EMG electrodes) + HW activation + Com Part 2 session + Files copy + Battery discharge + deactivation/stowage/ + Files downlink to ground Data Data stored on MARES and EPM Laptop Data downlink via EPM High Rate link Photo / Video Video required for Day 1 and Day 2 MARES

11. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 11 Sarcolab Science overview Investigation of the structural and functional changes in the muscles causing that phenomenon/characterization of reflex excitability of the calf muscles, before, during and after flight. Main Hardware MARES, EPM Laptop & Ext. HD, PEMS-2, CDL ADAS (+EMG electrodes), HRF Echo, consumables, EPM Rack MARES used with ankle configuration/ PEMS-2 connected to MARES/ CDL ADAS used for EMG data (synchronization) Muscle biopsy and MRI only during ground BDC Next subjects A. Kuipers, D. Petit (operator only) Implementation 3 sessions: FD 5-10 / FD 45 ± 15 days / R – 10 ± 8 days 2 days activities /session Crew time Day 1 : MARES deployment + HW set up+ activation + Battery charge + Ultrasound activity Day 2 : Subject equipment (PEMS + EMG electrodes) + HW activation + Sarcolab session + Files copy + battery discharge + deactivation/stowage/ + Files downlink to ground Data Data stored on MARES and EPM Laptop Data downlink via EPM High Rate link Photo / Video Video required for Day 1 and Day 2 MARES

12. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 12 Activity overview Trip issue on the EPM Laptop power line (which is common with the EPM Video unit) occurred during the NEUROSPAT Session (early summer 09) - SPR 357 Main Hardware EPM VIDEO UNIT Operator P. Nespoli Implementation Activity Plans: ■ EPM VU Power Connector J02 disconnection (Incr 25-26) ■ In case that failure is still present after connector disconnection: EPM Video Unit Power Harness stepwise interconnection tests ■ In case that VU is source of problem: EPM Video Unit Removal & Replacement (EPM VU FM2 upload on ATV-2; EPM VU FM-1 download on ULF7, TBC) Video Unit Replacement EPM MAINTENANCE

13. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 13 Overview ExperimentPre-flightPost-flight 3D-SPACE L-90d, L-60d, L-30dR+0d, R+1d, R+4, R+8 CARD Any time before L> R+60d IMMUNO Between L-30d/L-7dR+1d, R+7d, >R+30d NEUROSPAT L-90d, L-60d, L-30d.R+2, R+6, >R+15 (x2) PASSAGES >L-90dR+1d, >R+20d SOLO N/A>R+6M EDOS L-60d, L-30d R+1d (BM), R+2d (XCT), R+15d (BM), R+90d(BM & XCT), R+180d (BM & XCT), R+360d ±2w SPIN <L (x3) the same Wk R+1d, R+4d, R+9d: VVIS and Tilt test BDC

14. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 14 SARCOLAB ExperimentPre-flightPost-flight SARCOLAB Desired number of test subjects = 12 (minimum=10) Session A *: L-60±30 d R + 1 day Session B *: L-60 ± 30 days *: Min. 7 days between Session A & Session B R+2 to 4 days R+3 to 5 (+ 2 days) R+14 ± 2 days R+30 ± 5 days R+60 ± 7 days (#) (#) only if no full recovery in previous session BDC (2)

15. POIWG#29_CADMOS-Inc27-28 Overview I Jan. 25-27. 2011 I Huntsville, AL I Page 15 ACRONYMS ADASAmbulatory Data Acquisition SystemHRFHuman Research Facility BPBlood PressureMARESMuscle Atrophy Research and Exercise System CDLCardiolabMEEMMMulti ElectroEncephalogramme Mapping Module ECGElectrocardiogrammeMELFIMinus Eighty Degree Laboratory Freezer for ISS EEGElectroencephalogrammePCBAPortable Clinical Blood Analyzer EMGElectromyogrammePEMS-2Percutaneuous Electrical Muscle Stimulator 2 EOGElectrooculogrammeRCRefrigerated Centrifuge EPMEuropean Physiology Modules FacilitySWSoftware HDHard DiskVUVideo Unit