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Designing DNA Nanostructures to encapsulate and release proteins iGEM August 21, 2006 Katherine Fifer with Valerie Lau, Matthew Meisel, and Tiffany Chan.

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Presentation on theme: "Designing DNA Nanostructures to encapsulate and release proteins iGEM August 21, 2006 Katherine Fifer with Valerie Lau, Matthew Meisel, and Tiffany Chan."— Presentation transcript:

1 Designing DNA Nanostructures to encapsulate and release proteins iGEM August 21, 2006 Katherine Fifer with Valerie Lau, Matthew Meisel, and Tiffany Chan

2 Background: What is iGEM? iGEM - intercollegiate Genetically Engineered Machines Competition between about 40 schools in November Emphasis on biobricking parts to enable building of genetic circuits Biobricking parts makes them interchangeable (usually).

3 DNA Nanostructures Overview Can stick double helices together A few basic shapes have already been made A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron WILLIAM M. SHIH, JOEL D. QUISPE & GERALD F. JOYCE Nature 427, 618 ﾐ 621 (2004); doi:10.1038/nature02307

4 Motivation: For what purpose? Creating microenvironments Encapsulating, releasing proteins Specificity - arriving at certain targets Timed release A new drug delivery model? Thrombin Model Proof of principle

5 Motivation: Why DNA? The power of DNA Watson-Crick base pairing is enormously strong Self-assembly Highly programmable, designable Specificity

6 Scaffolded Oragami scaffold staples

7 Scaffolded Oragami

8 Progress Built a number of barrel designs Exciting EM Images Purifying Nanostructures (nearly there after 1 month of trials)

9 Exciting EM Images

10

11 EM Images (snakes on a grid) c5.0 barrel (10 nM), 0.7% uranyl formate Appear to be lining up end to end, probably because of the stain Images courtesy Shawn Douglas

12 EM Images Images courtesy Shawn Douglas

13 To be continued Protection assays Can a protein be protected from protease if attached inside the box Lid attachment Lid removal Strand displacement Protease or enzyme Protein or DNA ligand

14 Acknowledgements iGEM team - Valerie Lau, Matthew Meisel, Tiffany Chan, Perry Tsai, Lewis Hahn, Jeff Lau, Hetmann Hsieh, Zhipeng Sun, David Ramos iGEM TFs - Shawn Douglas, Nick Stroustrup, Chris Doucette iGEM advisers - Dr. William Shih, Dr. George Church, Dr. Pamela Silver, Dr. Alain Viel, Dr. Jagesh Shah, Dr. Radhika Nagpal PRISE Shawn Douglas Dr. William Shih

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