1. PowerLecture: Chapter 8 How Cells Release Stored Energy

2. Fig. 8-1, p.122 When Mitochondria Spin Their Wheels

3. p.123 When Mitochondria Spin Their Wheels

4.  Descendents of African honeybees that were imported to Brazil in the 1950s  More aggressive, wider-ranging than other honeybees  Africanized bee’s muscle cells have large mitochondria “Killer” Bees

5. Main Types of Energy-Releasing Pathways Aerobic pathways  Evolved later  Require oxygen Anaerobic pathways  Evolved first  Don’t require oxygen

6. start (glycolysis) in cytoplasm completed in mitochondrion start (glycolysis) in cytoplasm completed in cytoplasm Aerobic Respiration Anaerobic Energy- Releasing Pathways Fig. 8-2, p.124 Main Types of Energy-Releasing Pathways

7. Summary Equation for Aerobic Respiration C 6 H 12 0 6 + 6O 2 6CO 2 + 6H 2 0 glucose oxygen carbon water glucose oxygen carbon water dioxide dioxide

8. Overview of Aerobic Respiration CYTOPLASM Glycolysis Electron Transfer Phosphorylation Krebs Cycle ATP 2 CO 2 4 CO 2 2 32 water 2 NADH 8 NADH 2 FADH 2 2 NADH 2 pyruvate e - + H + e - + oxygen (2 ATP net) glucose Typical Energy Yield: 36 ATP e-e- e - + H + ATP H+H+ e - + H + ATP 2 4 Fig. 8-3, p. 135

9. The Role of Coenzymes  NAD + and FAD accept electrons and hydrogen  Become NADH and FADH 2  Deliver electrons and hydrogen to the electron transfer chain

10. Glucose In-text figure Page 126

11. Glycolysis Occurs in Two Stages Glycolysis Occurs in Two Stages  Energy-requiring steps ATP energy activates glucose and its six-carbon derivatives ATP energy activates glucose and its six-carbon derivatives  Energy-releasing steps The products of the first part are split into 2 three- carbon pyruvate molecules The products of the first part are split into 2 three- carbon pyruvate molecules ATP and NADH form ATP and NADH form

12. ATP 2 ATP invested ENERGY-REQUIRING STEPS OF GLYCOLYSIS glucose ADP P P P P glucose–6–phosphate fructose–6–phosphate fructose–1,6–bisphosphate DHAP Fig. 8-4b, p.127 Glycolysis

13. ATP ADP ENERGY-RELEASING STEPS OF GLYCOLYSIS NAD + P PGAL 1,3–bisphosphoglycerate substrate-level phsphorylation PiPi 1,3–bisphosphoglycerate ATP NADH P PGAL NAD + PiPi PPPP 3–phosphoglycerate PP 2 ATP PRODUCED ADP Fig. 8-4c, p.127Glycolysis

14. 2 ATP produced ATP ADP P substrate-level phsphorylation 2–phosphoglycerate ATP P pyruvate ADP PP 2–phosphoglycerate H2OH2OH2OH2O PEP Fig. 8-4d, p.127 Glycolysis

15. Energy-Requiring Steps 2 ATP invested Energy-Requiring Steps of Glycolysis glucose PGAL P P ADP P ATP glucose-6-phosphate P fructose-6-phosphate ATP fructose1,6-bisphosphate PP ADP Figure 8-4(2) Page 127

16. Energy- Releasing Steps ADP ATP pyruvate ADP ATP pyruvate H2OH2O P PEP H2OH2O P P 2-phosphoglycerate P ADP ATP P 3-phosphoglycerate ADP ATP P 3-phosphoglycerate NAD + NADH PiPi 1,3-bisphosphoglycerate PP NAD + NADH PiPi 1,3-bisphosphoglycerate PP PGAL P P Figure 8-4 Page 127

17. Glycolysis: Net Energy Yield Glycolysis: Net Energy Yield Energy requiring steps: Energy requiring steps: 2 ATP invested 2 ATP invested Energy releasing steps: 2 NADH formed 4 ATP formed Net yield is 2 ATP and 2 NADH

18. Second Stage Reactions Second Stage Reactions  Preparatory reactions Pyruvate oxidized into two-carbon acetyl units and carbon dioxide Pyruvate oxidized into two-carbon acetyl units and carbon dioxide NAD + is reduced NAD + is reduced  Krebs cycle The acetyl units are oxidized to carbon dioxide The acetyl units are oxidized to carbon dioxide NAD + and FAD are reduced NAD + and FAD are reduced

19. inner mitochondrial membrane outer mitochondrial membrane inner compartment outer compartment Fig. 8-6a, p.128 Second Stage Reactions Second Stage Reactions

20. Fig. 8-5b, p.128 Second Stage Reactions Second Stage Reactions

21. Preparatory Reactions pyruvate NAD + NADH coenzyme A (CoA) OO carbon dioxide CoA acetyl-CoA

22. Two pyruvates cross the inner mitochondrial membrane. outer mitochondrial compartment NADH FADH 2 ATP 2 6 2 2 Krebs Cycle 6 CO 2 inner mitochondrial compartment Eight NADH, two FADH 2, and two ATP are the payoff from the complete break- down of two pyruvates in the second-stage reactions. The six carbon atoms from two pyruvates diffuse out of the mitochondrion, then out of the cell, in six CO Fig. 8-6b, p.128

23. The Krebs Cycle Overall Products  Coenzyme A  CO 2  NADH  FADH 2  ATP Overall Reactants  Acetyl-CoA  NAD +  FAD  ADP and P i

24. Acetyl-CoA Formation acetyl-CoA (CO 2 ) pyruvate coenzyme A NAD + NADH CoA Krebs Cycle CoA NADH FADH 2 NADH ATP ADP + phosphate group NAD + FAD oxaloacetate citrate Fig. 8-7a, p.129 Preparatory Reactions

25. Krebs Cycle NAD + NADH =CoA acetyl-CoA oxaloacetatecitrate CoA H2OH2O malateisocitrate H2OH2O H2OH2O FAD FADH 2 fumarate succinate ADP + phosphate group ATP succinyl-CoA OO CoA NAD + NADH OO NAD + NADH  -ketoglutarate Figure 8-6 Page 129

26. Results of the Second Stage  All carbons in pyruvate end up in carbon dioxide  Coenzymes are reduced (they pick up electrons and hydrogen)  One molecule of ATP forms (2/Glucose)  Four-carbon oxaloacetate regenerates

27. Coenzyme Reductions during First Two Stages  Glycolysis2 NADH  Preparatory reactions 2 NADH  Krebs cycle 2 FADH 2 + 6 NADH  Total 2 FADH 2 + 10 NADH

28.  Occurs in the mitochondria  Coenzymes deliver electrons to electron transfer chains  Electron transfer sets up H + ion gradients  Flow of H + down gradients powers ATP formation Electron Transfer Phosphorylation

29. glucose GLYCOLYSIS pyruvate KREBS CYCLE ELECTRON TRANSFER PHOSPHORYLATION Fig. 8-8a, p.130 Phosphorylation

30. Fig. 8-8b, p.130 OUTER COMPARTMENT INNER COMPARTMENT Electron Transfer Chain ATP Synthase ATP H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ NADH + H + NAD + + 2H + FAD + 2H + FADH 2 2H + + 1/2 0 2 H2OH2O ADP + P i e-e- e-e- e-e- Phosphorylation

31. glucose glycolysis e–e– KREBS CYCLE electron transfer phosphorylation 2 PGAL 2 pyruvate 2 NADH 2 CO 2 ATP 2 FADH 2 H+H+ 2 NADH 6 NADH 2 FADH 2 2 acetyl-CoA ATP 2 Krebs Cycle 4 CO 2 ATP 36 ADP + P i H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ Fig. 8-9, p.131Phosphorylation

32. Importance of Oxygen  Oxygen accepts spent electrons from electron transfer chain… combines with H + to form water

33. Summary of Energy Harvest (per molecule of glucose)  Glycolysis 2 ATP formed by substrate-level phosphorylation 2 ATP formed by substrate-level phosphorylation  Krebs cycle and preparatory reactions 2 ATP formed 2 ATP formed  Electron transport phosphorylation 32 to 36 ATP formed 32 to 36 ATP formed

34. Energy Harvest Varies  NADH in cytoplasm cannot enter mitochondrion  delivers electrons to mitochondrial membrane  Membrane proteins shuttle electrons to NAD + or FAD inside mitochondrion  Electrons given to FAD yield less ATP than those given to NAD +

35.  686 kcal of energy are released  7.5 kcal are conserved in each ATP  When 36 ATP form, 270 kcal (36 X 7.5) are captured in ATP  Efficiency is 270 / 686 X 100 = 39 percent  Most energy lost as heat Efficiency of Aerobic Respiration

36. Fermentation Pathways Fermentation Pathways  Begin with glycolysis  Yield only the 2 ATP from glycolysis  Steps following glycolysis only regenerate NAD +

37. C 6 H 12 O 6 ATP NADH 2 acetaldehyde electrons, hydrogen from NADH 2 NAD + 2 2 ADP 2 pyruvate 2 4 energy output energy input glycolysis ethanol formation 2 ATP net 2 ethanol 2 H 2 O 2 CO 2 Fig. 8-10d, p.132 Alcoholic Fermentation

38. Fig. 8-10a, p.132 Alcoholic Fermentation

39. Fig. 8-10b, p.132 Alcoholic Fermentation

40. Fig. 8-10c, p.132 Alcoholic Fermentation

41. C 6 H 12 O 6 ATP NADH 2 lactate electrons, hydrogen from NADH 2 NAD + 2 2 ADP 2 pyruvate 2 4 energy output energy input glycolysis lactate fermentation 2 ATP net Fig. 8-11, p.133 Lactate Fermentation

42. Fig. 8-12, p.133 Lactate Fermentation

43. Anaerobic Electron Transport  Carried out by certain bacteria  Electron transfer chain in bacterial plasma membrane  Final electron acceptor is compound from environment (such as nitrate), not oxygen  ATP yield is low

44. FOOD fatsglycogen complex carbohydrates proteins simple sugars (e.g., glucose) amino acids glucose-6- phosphate carbon backbones NH 3 urea ATP (2 ATP net) PGAL glycolysis ATP 2 glycerol fatty acids NADHpyruvate acetyl-CoA NADHCO 2 Krebs Cycle NADH, FADH 2 CO 2 ATP many ATP water H+H+ e – + oxygen e–e– 4 ATP 2 Fig. 8-13b, p.135 electron transfer phosphorylation Alternative Energy Sources

45.  originally little oxygen in atmosphere  Earliest organisms - anaerobic  Later, noncyclic photosynthesis increased atmospheric oxygen Evolution of Metabolic Pathways

46. Processes Are Linked sunlight energy water + carbon dioxide PHOTOSYNTHESIS AEROBIC RESPIRATION sugar molecules oxygen

47. p.136a