1. Wednesday, February 17, 2016
What are we doing today?
Entry Task
review lactase and lactose
plan and prep Lactase Lab
Welcome back! Add Protein Reading to assignment sheet (#3, due 2/18) Watch this video on Lactose Digestion in Infants: Does your body make the enzyme lactase? How do you know?

2. Protein Reading Regular Honors
At the bottom of page one: cross out “nonfunctional” and write “non-working”
In question #4: cross out “non-functioning” and write “non-working”
Last paragraph of page one: cross out “functional” and write “working”
Last sentence of page one: cross out “nonfunctional” and write “non-working”
In question #5: cross out “non-functioning” and write “non-working”

3. Enzymes: A Fun Introduction Video
start at 2:23

4. Main Ideas from Video 1) each enzyme has an active site
2) enzymes are very specific
3) enzymes are recycled

5. Lactase Enzyme
Lactase is an enzyme produced by many organisms. It is found in the small intestine of humans and other mammals. Lactase is essential to the complete digestion of whole milk; it breaks down lactose, a sugar which gives milk its sweetness.

6. Drawing Carbohydrates
monosaccharide glucose (1 single sub-unit)
disaccharide lactose (glucose & galactose - 2 sub-units)
Glucose Galactose

7. Lactase Enzyme breaks disaccharide (lactose) into two monosaccharides (glucose and galactose)
Don’t forget water!
Wow! Look at that shape!

8. lactose + water glucose + galactose
Lactase Enzyme breaks disaccharide (lactose) into two monosaccharides (glucose and galactose)
lactase
lactose + water glucose + galactose

9. Don’t forget water!

10. Enzymes work because the active site matches the substrate.

11. Lactase works inside your small intestine.
Human body temperature is 37 °C or 98.6°F
Human small intestine acidity is pH 6.7

12. Lactase animation Lactase enzyme galactose glucose
Have students narrate where this happens, and what is happening. Honors: hydrolysis!
galactose
glucose
Lactase enzyme

13. Lactase Enzyme Lab
Purpose: to determine how environmental factors affect the ability of an enzyme to do it’s job
We will use glucose test strips that only test for ______________.

14. You could test temperature or pH. Let’s look at temperature first!
lactase exposed to boiling temperature
lactase exposed to room temperature
Lactase exposed to freezing temperature
Will these lactase solutions function? (break down lactose into glucose and galactose?)

16. You could test temperature or pH. Let’s look at pH now!
lactase in acidic solution
(pH 2)
lactase in neutral solution
(pH 7)
lactase in basic solution
(pH 9)
Will these lactase solutions function? (break down lactose into glucose and galactose?)

17. The pH Scale

18. Lactase Lab Planning Template
Before you leave today, you will finish:
When you are done:
update assignment sheet
the first page
get missing work (if you were absent or late)
Variables
Research Question
do Protein Reading
Hypothesis
Looking for a special project? See Ms. Anne
Materials and Procedure
Be sure to:
work with all your lab group members
get signed off in three places

19. Temperature
Three enzyme solutions (one lactase tablet mL of water):
frozen then thawed
room temperature
boiled then cooled

20. pH
Three enzyme solutions:
acidic – pH 1
neutral – pH 7
basic – pH 14

21. Thursday, February 18, 2016 What are we doing today? Entry Task
collect data for Lactase Lab
graph lab group data
Get a stamp for Protein Reading. Find a friend who is testing another manipulated variable and tell them your research question and hypothesis. Be prepared to share out your friend’s research question and hypothesis in three minutes.

22. Lactase Lab Scoring Rubric
What is it?
When would you like it? Class choice!
a guide for writing your Lactase Lab Conclusion next Tuesday (similar to Gummy Bear Lab Conclusion, will count as a summative assessment)
a checklist for what Ms. Wang and Ms. Anne will be grading you on
today at the end of class
tomorrow
Monday
Tuesday (day of conclusion writing)

23. Measuring Glucose Concentration
put five drops of lactase solution into (nine) wells
add five drops of milk
wait five minutes
dip test strip in milk and stir for 30 seconds (12 wells)
remove the strip and wait three minutes
compare your glucose stick to the chart below

24. When you are done collecting data…
calculate your lab group’s averages
start graphing your data
x-axis label and units
y-axis label and units
appropriate scale
graph title
three data points
be prepared to present your neat and accurate graph tomorrow

25. Friday, February 19, 2016 Talk with your lab group:
What are we doing today?
Entry Task
present lab group data
Toothpickase Enzyme Activity
Talk with your lab group:
What does your Lactase Lab data mean?
Why do you think you got the results you did?
Be prepared to share out in three minutes.

26. Data Presentations Why are we presenting data? Be sure to:
Scientists share their data with other scientists and with citizens
inform others
build off each other’s research
Talking through your ideas and explaining to others will help you learn
We, our Biology class, are a learning community!
all lab group members should speak
speak loudly and clearly so others can hear
explain your group’s graph
manipulated variable
responding variable
data values
reasoning

27. Toothpickase Enzyme Activity
Read the Instruction sheet (class copy, return to Ms. Anne)
Activity 1: designate two toothpickases, one recorder, one timer
Activity 2: switch roles
Throw away toothpicks
Turn into inbox when done (Ms. Anne will give you a participation grade)
Done?
turn in/finish late work
start a rough draft conclusion (CER) for Lactase Lab
looking for a special project? See Ms. Anne
4th period: help Ms. Anne dump ice water, put away tubs + toothpicks + bowls