1. Hot Water Extraction of Woodchips and Utilization of the Residual Chips and Wood Extracts Date 2/2/2011 Biomass Program IBR Platform – DEFG607G087004 Thomas E. Amidon State University of New York – College of Environmental Science and Forestry (SUNY ESF) This presentation does not contain any proprietary, confidential, or otherwise restricted information

2. 2 Quad Chart Overview Project start date –October 1, 2007 Project end date –September 30, 2010 –Searching funding to continue to next stage 100% completion Project is complete Cost and schedule on track Project completed as planned Total project funding –DOE share: $2,201625 –Cost share: $555,175 Funding received by Fiscal Year FY07 $750,000 FY08 $738,000 FY09 $713,625 Timeline Budget Project Development One co-Pi is actively coordinating with all parties to meet overall project goal Intellectual properties developed PI overall management Project Participants

3. Spend Plan 3

4. Project Overview 4 Pilot Development Commercialization Actively seeking funding to continue - $10 M estimated need

5. 5 Approach This is a basic research project Funds distributed to two individual researchers, while retaining direct control over 70% of the funds on a need basis Research contributions were voluntary from co-PI’s Extensive bench scale experiments. Some pilot scale extraction experiments conducted (to provide extracts / hydrolysates for other objectives) There is no “no-go” decision / gate designed.

6. 6 Technical Accomplishments/ Progress/Results

7. 7 No pretreatment –Only size reduction (by chipping) –Hot-water extraction replaces pretreatment No detoxification –Promoting separation –Membrane technology Multiple Product Stream –Incremental deconstruction –Optimizing conversion and fraction

8. 8 Technical Accomplishments/ Progress/Results Extraction: Mass Balance

9. Technical Accomplishments/ Progress/Results Extraction: PH / Kinetics 9

10. Technical Accomplishments/ Progress/Results Extraction 10

11. Technical Accomplishments/ Progress/Results Extract Properties 11

12. Technical Accomplishments/ Progress/Results Extract Properties 12

13. Technical Accomplishments/ Progress/Results Extract Hydrolysis Enzymatic 13

14. Technical Accomplishments/ Progress/Results Extract Hydrolysis Enzymatic 14

15. Technical Accomplishments/ Progress/Results Extract Hydrolysis Enzymatic 15

16. Technical Accomplishments/ Progress/Results Extract Hydrolysis: dilute acid 16

17. Technical Accomplishments/ Progress/Results Extract Hydrolysis: dilute acid 17

18. Technical Accomplishments/ Progress/Results Extract Hydrolysis: dilute acid 18

19. Technical Accomplishments/ Progress/Results Extract Hydrolysis: dilute acid 19

20. Technical Accomplishments/ Progress/Results Extract Hydrolysis & Aromatic/lignin removal 20

21. Technical Accomplishments/ Progress/Results Extract concentration and hydrolysate fractionation 21

22. Technical Accomplishments/ Progress/Results Extract concentration and sugar stream purification 22

23. Technical Accomplishments/ Progress/Results Extract concentration and sugar stream purification 23

24. Technical Accomplishments/ Progress/Results Extract hydrolysate utilization: ethanol fermentation 24

25. Technical Accomplishments/ Progress/Results Extract hydrolysate utilization: ethanol fermentation 25

26. Technical Accomplishments/ Progress/Results Extract hydrolysate utilization: ethanol fermentation 26

27. Technical Accomplishments/ Progress/Results Residual biomass utilization: fiber quality 27

28. Technical Accomplishments/ Progress/Results No waste generation from pretreatment No waste generation from detoxification Recovery of lignin / aromatics for high value applications Ethanol fermentation successful with Modified E. coli fbr5 (genetic engineered strain) Modified P. Stipitis (generic strain) No detoxification High yield (~40 g/g) PHA fermentation successful Residual biomass improved for high value application (other than fuel chemicals) – Pulp, reconstituted wood products etc. Ready for next step: pilot demonstration / commercial facility 28

29. 29 Relevance Woody biomass is the most abundant renewable carbon/energy source in nature Production of bio-based chemicals, liquid fuel and materials from mixed sugars of biomass Reduce cost and eliminate waste products (no pretreatment or detoxification) Co-products significantly increase revenue – 2X+ Technology has been licensed to ABS for commercialization AF&PA has proposed commercializing at a US Pulp Mill

30. 30 Success Factors and Challenges Success Factors: High Yield of ethanol from mixed sugars of biomass Success Factors: bio-based chemicals and liquid fuel production without pretreatment / detoxification Water based process with high value co-products Challenges: Funding to commercialization Efficient commercial production is key technical/engineering challenge

31. Benefits and Expected Outcomes 31 Production of biochemical's, liquid fuel and materials from woody biomass Fermentation using generic fermentors (reducing public concern) No Pretreatment or detoxification and no external waste products creation from them Faster commercialization resulting from increased revenues of co- products Pellet Properties and pulping effects assist commercialization

32. 32 Summary Conversion of biomass to chemicals, energy and materials without pretreatment and detoxification reduces waste production Use of generic fermentors for bioconversion, reduces public concern on mass production Pilot scale extraction experiments successful Woody biomass use enables long-term sustainability without food competition