1. 8th April 2014 An Innovative Approach to Improved Pig Health and Farmer Profitability THE PROBIOTIC CONCEPT The Probiotic Concept Composed by:Eric van den Heuvel Delivered by: Dan Kirschner info@pro-b.ie

2. *What’s new/innovative *Principles behind it *Product Range/ Application *Benefits Farmer Profitability – profit Centre Pig Welfare *Case Study *Questions Agenda The Probiotic Concept

3. * Meet my family Eric van den Heuvel (50 yrs) Married with Ine (51 yrs) 3 Children Gert (24 yrs) Marieke (18 yrs) Eveline (12 yrs) The Probiotic Concept

4. GROTE HEIDE 500 sows, built in 1992 Nistelrode The Probiotic Concept

5. Innovation in Pig Farming The Probiotic Concept Eric van den Heuvel Dan Kirschner

6. *Control pathogens – Reduced mortality *Improved Food to Weight conversion – Improved Profitability *Reduced Odours – Better Environment *Reduced Antibiotics – Improve quality Using Probiotic as a Cleaning Agent to: The Probiotic Concept

7. Principle behind this Probiotic Concept Or How does it work? The Probiotic Concept Eric van den Heuvel Dan Kirschner

8. Three Core Products Desired and undesired bacteria Adding desired bacteria The Probiotic Concept Cleaner: Detergent + Probiotic bacteria Spray: Probiotic bacteria only Drinking lines cleaner: Probiotic bacteria only In the house In the drinking lines

9. Chemical detergent AntibioticsDisinfection Bacteria are being killed Old system: Result: Resistance of pathogenic bacteria!!! For example MRSA, ESBL, Ehec Bacteria The Probiotic Concept

10. In the farmIn the drinking lines We should start controlling bacteria The Probiotic Concept Biofilm

11. Desired and undesired bacteria Most resistant bacteria remain Lot’s of space to grow What happens with the use of disinfectants and/or the use of antibiotics? The Probiotic Concept

12. Control Bacteria with the Probiotic Concept Desired and undesired bacteria Adding desired bacteria No room for undesired bacteria to develop The Probiotic Concept

13. Cleaning the drinking lines: Dosing 1:10,000 all drinking lines

14. Cleaning the house and sow: - remove bulky dirt - foam and wait for a few minutes - wash with high pressure - normally no need for disinfecting - wash the sow (M v A?) Note: Foam without protective clothing The Probiotic Concept

15. Maintain healthy microflora by spraying the bacteria regularly throughout the farm. Frequent may vary!

16. Benefits The Probiotic Concept Eric van den Heuvel Dan Kirschner

17. The Probiotic Concept Visual Results Further more, Hardly No Smell (sorry can’t demonstrate)!

18. Floor and wall measurements Stafylococcus Aureus: Date:2-3-20106-5-20109-9-2010 CFU2010 CFU5250 CFU1570 CFU6610 CFU10010 CFU: Colony Forming Units The Probiotic Concept

19. Barn: Room:3 Report: Animal: Weaned pig Sample taken from: Nipple Description: Flushed:yes Total bacteria count 60.000CFU/ml Yeast0CFU/ml Mould 0CFU/ml Enterococcus0CFU/ml Stafylococcusnot shownCFU/ml Streptococcus not shown CFU/ml MRSA not shown CFU/ml E-coli not shown CFU/ml Clostridium not shown CFU/ml BRMO not shown CFU/ml Kewi Services Sampledate: 05/08/2011 Date Result: 22/08/2011 The Probiotic Concept Drinking lines lab results

20. Technical Results Grote Heide: 500 sows Piglets born alive per acouchement Pigs weaned per sow/year Discards before weaning Discards after weaning 200814,530,016,4%1,5% 200914,630,814,6%1,3% 201014,830,814,9%1,6% 201114,731,012,8%2,0% The Probiotic Concept In addition we note improvement in weight gain and FCR

21. The Probiotic Concept

22. Better health for human, animal and environment A total concept with many advantages Lower risk to get MRSA and other zoonoses Hardly any antibiotics needed No disinfectants needed Less smell Less dust Reduction of ammonia Less endotoxins The Probiotic Concept

23. Happy Animals Yield More The Probiotic Concept Managing health with respect for human, animal and environment

24. Where else is the Probiotic Concept implemented The Probiotic Concept Chicken farms Nurseries Trains Gyms Hotels

25. Case Study The Probiotic Concept Eric van den Heuvel Dan Kirschner

26. * Meet my family Eric van den Heuvel (50 yrs) Married with Ine (51 yrs) 3 Children Gert (24 yrs) Marieke (18 yrs) Eveline (12 yrs) The Probiotic Concept

27. * October 2003 daughter Eveline planned to have 2 nd open heart surgery *Due to life threatening situation Eveline had to be operated 3 months later *With pre-operation check: MRSA positive *Result: being sent home and no operation The Probiotic Concept

28. The beginning of the MRSA problems Wait, wait, wait… The Probiotic Concept

29. We get two weeks to become MRSA free Wash with Hibiscrub 3x per week Nasal ointment bactroban 2x per day Mouth/throat wash corsodyl 4x per day The Probiotic Concept

30. Finally 14 days later she can be operated The Probiotic Concept

31. STRAIN DETERMINATION Every hospital has its own strain. The strain of Eveline was not traceable! Where does this MRSA strain come from? 1 st name: NT-MRSA Microbiologist: Dr. Andreas Voss The Probiotic Concept

32. Samples tested for MRSA. December 2004 25 pig farmers from study group 5 positive. This is 20% Dutch population lower than 0,1% ! Conclusion: There seems to be a connection between MRSA prevalence and pigs. The Probiotic Concept

33. NT MRSA Pig MRSA Animal related MRSA ST 398 The Probiotic Concept

34. MRSA Measurements in the family PERSON Mrt '10 Jun '10 Okt '10 Mrt '11 Aug '11 Okt '11 Jan ‘12 Apr ‘12 Jul ‘12 Okt ‘12 Jan ‘13 Mei ‘13 Aug ‘13 Nov ’13 ERIC POS NEG POSNEG POS INE POS NEG POSNEG GERT POS NEG POSNEG MARIEKE NEG EVELINE POSNEG The Probiotic Concept

35. Thank you for your attention The Probiotic Concept Eric van den Heuvel Dan Kirschner info@pro-b.ie