1. Mosquitoes as Pests and Vectors of Disease: A Collaborative Effort between Natrona County Health Department and University of Wyoming/Casper College Skye Napolitano, A.S., Kelsey Deus, Ph.D. University of Wyoming/Casper College

2. How many mosquitoes are here? 2

3. 3

4. …How about now? 4

5. Abstract Mosquito population composition in Natrona Co. Testing for West Nile Virus (WNV), Western Equine Encephalitis (WEE), and St. Louis Encephalitis (SLE) 5

6. Introduction Every state has reported WNV presence 692 confirmed human infections in WY 2002-2010 WEE reported in neighboring states Birds are only host reservoirs 6

7. Methods 7

8. Collecting Larval collection with standard mosquito dipper Site for larvae dried up (Shooter’s Complex) Adult collection with New Jersey light traps 8

9. Testing VecTest Vortex mixer Pulverization Dipstick assays 9

10. Preservation Low population numbers did not merit a lab colony Collection from field Point punch and #3 insect pins used to mount 10

11. Procedure 8 sites in Natrona Co. Convenience sample Systematic sampling Complete and partial submersion Adults collected with New Jersey light traps 11

12. Results Adult Population Fluctuation Primarily composed of aedes vexans, aedes dorsalis, culex tarsalis, culex pipiens, culiseta inornata 12

13. Testing WNV, WEE, & SLE Negative Culex and Aedes Distended thoraces CDC dry ice traps 13

14. Discussion Unusual season: ▫ Fire ▫ Temperature Larval vs. Adult populations Wealth of data 14

15. Wildfire List IncidentTypeUnitLocationStatusAcresUpdated North Buffalo FireWildfire Bridger - Teton National Forest Wyoming, USAActive28,60051 min. ago GileadWildfireBighorn National ForestWyoming, USAActive8,18951 min. ago Fontenelle FireWildfire Bridger - Teton National Forest Wyoming, USAActive64,22059 min. ago Chall CreekWildfire Bridger - Teton National Forest Wyoming, USAActive6871:01 hrs. ago Alpine Lake FireWildfireWind River AgencyWyoming, USAActive43,03218 hrs. ag Horsethief CanyonWildfire Bridger - Teton National Forest Wyoming, USAActive3,37320 hrs. ago Bear Cub FireWildfire Bridger - Teton National Forest Wyoming, USAActive6,5004 days ago Cygnet ComplexWildfireYellowstone National ParkWyoming, USAActive4,3965 days ago Sheep Herder HillWildfireWyoming State ForestryWyoming, USAActive15,5567 days ago Arapaho FireWildfire Medicine Bow National Forest & Thunder Basin National Grassland Wyoming, USAActive98,1158/24/2012 Alpine LakeWildfireShoshone National ForestWyoming, USAInactive1,3428/15/2012 Little BoxelderWildfireWyoming State ForestryWyoming, USAInactive6,5018/15/2012 Fan 1 and Fan 2 FiresWildfireYellowstone National ParkWyoming, USAInactive28/12/2012 Dewdrop FireWildfireYellowstone National ParkWyoming, USAInactive258/12/2012 Ferris FireWildfireBLM High Desert DistrictWyoming, USAActive8,7978/9/2012 SeminoeWildfireBLM High Desert DistrictWyoming, USAInactive3,6828/1/2012 Pilot HillWildfire Medicine Bow National Forest & Thunder Basin National Grassland Wyoming, USAInactive17/20/2012 Squirrel CreekWildfire Medicine Bow National Forest & Thunder Basin National Grassland Wyoming, USAActive10,9217/20/2012 Sawmill CanyonWildfireWyoming State ForestryWyoming, USAInactive14,1857/19/2012 Blacktail FireWildfireYellowstone National ParkWyoming, USAInactive157/13/2012 Index CreekWildfireShoshone National ForestWyoming, USAInactive2237/12/2012 Oil CreekWildfireWyoming State ForestryWyoming, USAInactive62,3187/10/2012 Russells Camp FireWildfireMedicine Bow National Forest & Thunder Basin National Grassland Wyoming, USAInactive5,4667/1/2012 15

16. Airport Holding Pond 16

17. Evansville Dump 17

18. Holbrook 18

19. K. Waters 19

20. Miladinovich 20

21. P. Blair 21

22. Shooter’s Complex 22

23. W. Waters 23

24. References 24 Centers for Disease Control and Prevention, Division of Vector-Borne Diseases. (2011). Background: virus history and distribution. Retrieved from website: http://www.cdc.gov/ncidod/dvbid/westnile/background.htmhttp://www.cdc.gov/ncidod/dvbid/westnile/background.htm Centers for Disease Control and Prevention, Division of Vector-Borne Diseases 2. (2009).Vertebrate Ecology. Retrieved from website: http://www.cdc.gov/ncidod/dvbid/westnile/birds&mammals.htmhttp://www.cdc.gov/ncidod/dvbid/westnile/birds&mammals.htm Domingo, C., Patel, P., Linke, S., Achazi, K., & Niedrig, M. (2011). Molecular diagnosis of flaviviruses. Future Virology, 6(9), 1059-1074. Retrieved from http://search.proquest.com.jaws.caspercollege.edu/docview/890053122/abstract?source=fedsrch&accountid=296 44. http://search.proquest.com.jaws.caspercollege.edu/docview/890053122/abstract?source=fedsrch&accountid=296 Kwan, J.L., Kluh, S., Madon, M.B., & Reisen, W.K., (2010). West Nile virus emergence and persistence in Los Angeles, California, 2003-2008. American Journal of Tropical Medicine and Hygiene, 83, 400-412. Mendoza F., Ibáñez-Bernal S., & Cabrero-Sañudo F.J. (2008). A standardized sampling method to estimate mosquito richness and abundance for research and public health surveillance programmes. Bulletin of Entomological Research, 98, 323-332 doi:10.1017/S0007485308005701 Wilder-Smith, A., Earnest, A., Tan, S. B., Ooi, E. E., & Gubler, D. J. (2010). Lack of association of dengue activity with haze. Epidemiology and Infection, 138(7), 962-7. doi: 10.1017/S0950268810000683 Wyoming Department of Health. (2012). WNV Activity in Wyoming by Year. Retrieved from website: http://www.health.wyo.gov/phsd/skeeter/WNVactivity.html http://www.health.wyo.gov/phsd/skeeter/WNVactivity.html

25. Questions? 25