1. Staphylococcus spp. 2011274010 방소연 자료조사 2011274016 서유진 자료조사
이은송 PPT제작
은경남 자료조사
손주영 PPT제작
황세미 발 표

2. Contents Gram positive VS Gram negative Classification Identification
Characteristic
S.aureus
MRSA
Disease
Other staphylococci
Prevention and Treatment
Q&A
Quiz

3. Gram-positive vs Gram-negative

4. Classification

5. Identification Catalase test Coagulase test , mannitol salt agar
S. aureus : positive

6. Characteristic spherical cells (1 m) arranged in grapelike clusters
encapsulated
Gram positive
Non-motile, non-spore forming
catalase (+)
Normal flora of humans found on nasal passages, skin, mucous membranes

7. S. aureus Large, round, opaque colonies Facultative anaerobe
withstand high salt, extremes in pH, and high temperature
Hemolysis
a troublesome hospital pathogen

8. Enzymes

9. Toxins

10. MRSA (methicillin-resistant Staphylococcus aureus)
MRSA is hard to treat because it cannot be killed by many common antibiotics. MRSA can infect any part of the body

11. Disease Localized cutaneous infections
Carbuncle Impetigo folliculitis
Diseases resulting from direct organ invasion
Osteomyelitis, pneumonia, acute endocarditis,
septic arthritis, meningitis, skin infection

12. Disease Diseases caused by exotoxin release 1. Food intoxication
2. Staphylococcal scalded skin syndrome (SSSS)
3. toxic shock syndrome (TSS)

13. Food poisoning Enterotoxin Food borne transmission
Suddenly vomit, diarrhea,
No fever, rash

14. Staphylococcal scalded skin syndrome
Newborn
Exfoliative toxin
Dequamation of the epidermidis

15. Toxic Shock Syndrome
Initially associated with retained highly absorbent tampon
Staphylococcus aureus → exotoxin production
Fever, vomiting, diarrhea → renal failure and shock
Generalized rash followed by desquamation of skin

16. Other Staphylococci S. epidermidis 7.5% NaCl
Coagulase – negative staphylococci (CNS)
Mannitol fermentation X
S. epidermidis
7.5% NaCl
Normal habitat in the skin & mucous membranes
endocarditis, bacteremia, urinary tract infections
S. hominis
in skin areas high in apocrine gland
S. capitis
the scalp, face, external ear

17. Other Staphylococci S. saprophyticus
Clinical Disease : Acute Urethral Syndrome
Cystitis
Pyelonephritis
Urinary tract infetion
Diagnosis : non-mannital fermentor,
Novobiocin resistant

18. Prevention and Treatment
-hand washing
-adequate cleansing of surgical incisions
and burns
Treatment
- antibiotics(penicillin, methicillin, vancomycin)
- surgically perforated and cleared of pus

19. Q&A

20. Quiz

21. 1. Which are not related with Staphylococcus aureus?
① Oxidase positive
② Nitrate reductase
③ Coagulase producing
④ Mannitol degradation
⑤ DNA degradation

22. 3. Which enzyme produced by Staphylococcus aureus causes invasion of skin and subcutaneous tissue? ① Coagulase ② Hyaluronidase ③ Lipase ④ Fibrinolysin ⑤ Nuclease

23. 2. Which are not characters of Staphylococcus aureus
2. Which are not characters of Staphylococcus aureus? ① They develop well in high salt(NaCl 7.5%). ② They cause beta-hemolysis in sheep blood agar. ③ They degradate glucose only oxidatively. ④ They produce yellow colony. ⑤ They can be curtured in mannitol salt agar.

24. 4. What is not the extracellular enzyme that is produced by Staphylococcus aureus? ① Coagulase ② Lipase ③ Hyaluronidase ④ DNase ⑤ Oxidase

25. 5. Which are not related with the toxin that cause food poisoning
5. Which are not related with the toxin that cause food poisoning? ① It is enterotoxin. ② It is Exotoxin. ③ It is destroyed in 100℃ for 30 minutes. ④ It is produced by pathogenic Staphylococci. ⑤ It is occurred in 2 to 6 hours after eating food.

26. 6. What is the disease that caused by Staphylococcus aureus
6. What is the disease that caused by Staphylococcus aureus? a) Skin disease just like boil b) Pneumonia c) Food poisoning d) Osteomyelitis ① a, b, c ② a, c ③ b, d ④ d
⑤ a, b, c, d

27. 7. Which bacteria is positive to coagulase
7. Which bacteria is positive to coagulase? a) Staphylococcus aureus b) Staphylococcus saprophyticus c) Staphylococcus hominis d) Staphylococcus aureus ① a, b, c ② a, c ③ b, d ⑤ a, b, c, d
④ d