2. K. Martin KINGS AND QUEENS!!!

3. K. Martin RouterModesWANEncapsulationWANServicesRouterBasicsRouterCommands 100 200 300 400 500RouterModesWANEncapsulationWANServicesRouterBasicsRouterCommands 100 200 300 400 500 1 DNA Tech Basics 1 DNA Tech Techniques Forensics Stem Cells Cloning Gene Therapy 100 200 300 400 500 100 200 300 400 500 ► ► ► F i n a l Question ◄ ◄ ◄

4. K. Martin Answer Process where humans select the traits that are to be bred for in a particular species. DNA Tech Basics 100 ARTIFICIAL SELECTION or SELECTIVE BREEDING 100 200 300 400 500

5. K. Martin Answer KHOLARABI, BROCCOLI, BRUSSELS SPROUTS, CABBAGE, CAULIFLOWER, KALE Name one of the six vegetables that was bred for from wild mustard. DNA Tech Basics 200 100 200 300 400 500

6. K. Martin Answer 100 200 300 400 500 HYBRID The offspring of two different species. DNA Tech Basics 300

7. K. Martin Answer 100 200 300 400 500 BIOETHICS What field of biology studies the implications involved behind the decisions whether something is right or wrong? DNA Tech Basics 400

8. K. Martin Answer 100 200 300 400 500 HUMAN GENOME PROJECT (HGP) Completed in 2003, this accomplishment sequences all 3.2 billion base pairs found in human DNA. DNA Tech Basics 500

9. K. Martin Answer 100 200 300 400 500 POLYMERASE CHAIN REACTION (PCR) The process of alternating temperatures of a DNA sample to create billions of copies. DNA Tech Techniques 100 200 300 400 500

10. K. Martin Answer 100 200 300 400 500 GEL ELECTROPHORESIS This technique separates molecules based on size. DNA Tech Techniques 200

11. K. Martin Answer 100 200 300 400 500 RESTRICTION ENZYMES These proteins cut DNA at specific recognition sequences. DNA Tech Techniques 300

12. K. Martin Answer 100 200 300 400 500 VIRUSES and PLASMIDS What are the two main types of vectors used in DNA technology? DNA Tech Techniques 400

13. K. Martin Answer 100 200 300 400 500 FLUORESCENCE IN SITU HYBRIDIZATION (FISH) What technique uses a probe to light up the gene? DNA Tech Techniques 500

14. K. Martin Answer 100 200 300 400 500 SHORT TANDEM REPEATS (STR) What is the region between the genes called where much of the variation between individuals takes place? Forensics 100

15. K. Martin Answer 100 200 300 400 500 13 How many STRs does the FBI use to determine the likeliness of DNA profiles? Forensics 200

16. K. Martin Answer 100 200 300 400 500 Male 1 Who’s the father? Forensics 300

17. K. Martin Answer 100 200 300 400 500 TACGTC What are the first 6 nucleotides in this sequence of DNA? Forensics 400

18. K. Martin Answer 100 200 300 400 500 1.5% What percentage of human DNA codes for proteins? Forensics 500

19. K. Martin Answer 100 200 300 400 500 TOTIPOTENT What type of cells can develop into any cell type? Stem Cells 100

20. K. Martin Answer 100 200 300 400 500 INDUCED PLURIPOTENT STEM CELLS, (iPS CELLS) When somatic cells are turned back into stem cells, what type of cell do they become? Stem Cells 200

21. K. Martin Answer 100 200 300 400 500 INNER CELL MASS (ICM) What region of the blastula/blastocyst do adult stem cells come from? Stem Cells 300

22. K. Martin Answer 100 200 300 400 500 ECTODERM, MESODERM, ENDODERM What are the three germ layers of a gastrula? Stem Cells 400

23. K. Martin Answer 100 200 300 400 500 MULTIPOTENTCY What type of potency can form the least amount of differentiated cell types but is still a type of stem cell? Stem Cells 500

24. K. Martin Answer 100 200 300 400 500 GENE CLONING What type of cloning uses recombinant DNA vectors? Cloning 100

25. K. Martin Answer 100 200 300 400 500 THE NUCLEAR DONOR In the creation of Dolly, what sheep is genetically identical to her? Cloning 200

26. K. Martin Answer 100 200 300 400 500 SOMATIC CELL NUCLEAR TRANSFER (SCNT) or REPRODUCTIVE CLONING What process created Dolly? Cloning 300

27. K. Martin Answer 100 200 300 400 500 THERAPEUTIC & REPRODUCTIVE CLONING What two types of cloning use the genetic material of a somatic cell and places it into a donor egg cell. Cloning 400

28. K. Martin Answer 100 200 300 400 500 NONE In what country is human cloning legal? Cloning 500

29. K. Martin Answer 100 200 300 400 500 DNA PROBES What are ssDNA molecules that detect certain genes called? Gene Therapy 100

30. K. Martin Answer 100 200 300 400 500 IN VITRO FERTILIZATION (IVF) What is it called when a sperm fertilizes an egg in a test tube or petri dish? Gene Therapy 200

31. K. Martin Answer 100 200 300 400 500 RADIOACTIVE ssDNA & FLUORESCENT ssDNA What are the two main types of probes used in genetic therapy? Gene Therapy 300

32. K. Martin Answer 100 200 300 400 500 PREIMPLANTATION GENETIC DIAGNOSIS (PGD) What is it called when one cell of a morula is tested for a genetic disorder? Gene Therapy 400

33. K. Martin Answer 100 200 300 400 500 RNAi What molecule can intercept an mRNA molecule before it reaches the ribosome to essentially “turn off” a gene? Gene Therapy 500

34. K. Martin Answer CHICKS WITH DYED FEATHERS, BREEDING GIANT PANDAS, PUREBRED DOGS, SHEARED WOOLY SHEEP, VEAL, ANY OTHERS THAT APPLY What bioethical issue discussed in class does not need DNA technology? Final Question 100 200 300 400 500