1. Lab 1.41 GelScape and GelBank David Wishart University of Alberta Edmonton, AB david.wishart@ualberta.ca

2. Lab 1.42 GelScape Web-enabled gel viewing and annotation tool Allows users to post, share and compare gels in a free, platform independent manner A Java Applet with extensive Perl and HTML Tested and operable on most platforms (UNIX, Linux, Windows, MacOS X) using most browsers (IE and Netscape > 4.5) Conceptually aligned with web mail Developed by Nelson Young & Casper Chang

3. Lab 1.43 GelScape Reference www.gelscape.ualberta.ca

4. Lab 1.44 GelScape Supports... 1D and 2D gel image uploading (gif and jpg) from local machine Non-local (server-side) storage of annotated gels Image resizing (zooming) Spot marking and unmarking Spot annotation (via Swiss Prot ID, mass fingerprint, hand annotation)

5. Lab 1.45 GelScape Supports... MW and pH grid drawing and dragging Spot edge detection and spot integration Interactive, image map spot annotation display Gel comparison (overlay, transparency) Gel legend display Image saving, image uploading (to GelBank), image printing (preview)

6. Lab 1.46 http://www.gelscape.ualberta.ca:8080/index.html

7. Lab 1.47 Sign in...

8. Lab 1.48 First Screen...

9. Lab 1.49 Select a Gel Image...

10. Lab 1.410 Or Select Pre-existing Image

11. Lab 1.411 Annotate/View Mode

12. Lab 1.412 3 Kinds of Annotation Modes

13. Lab 1.413 Swiss-Prot Mode (Fast)

14. Lab 1.414 Mass Fingerprint (Fast)

15. Lab 1.415 Click on a Marked Spot

16. Lab 1.416 Setting Axes, pH & MW Grids

17. Lab 1.417 If You Change Grid – Save!

18. Lab 1.418 Zooming

19. Lab 1.419 Zooming

20. Lab 1.420 Peak Picking/Integration

21. Lab 1.421 Peak Picking/Integration

22. Lab 1.422 Toggle the Spot Box

23. Lab 1.423 Click Box – Adjust Threshold

24. Lab 1.424 Viewing A Gel Legend

25. Lab 1.425 Printing a Hard Copy

26. Lab 1.426 Saving Gels (3 Modes)

27. Lab 1.427 Comparing Gels (3 Modes)

28. Lab 1.428 Gel Warping

29. Lab 1.429 Gel Warping

30. Lab 1.430 GelBank A public, web-accessible database that stores annotated gels produced from GelScape Accessible directly from GelScape Gel Images are stored as clickable HTML Image Maps Supports gel querying from level of organism, organ/tissue, Swiss-prot ID and protein name Relatively few entries so far

31. Lab 1.431 Federated 2D Gel Databases Remotely queryable via the web Attainable through SWISS-PROT search Linked to other 2D databases via web Image mapped 2D gel spots to support graphical image query Directly reachable from within 2D gel analysis software Appel, R.D. et al. Electrophoresis 17: 540-546 (1996)

32. Lab 1.432 GelBank Remotely queryable via the web Attainable through SWISS-PROT search Linked to other 2D databases via web Image mapped 2D gel spots to support graphical image query Directly reachable from within 2D gel analysis software Appel, R.D. et al. Electrophoresis 17: 540-546 (1996)

33. Lab 1.433 GelBank

34. Lab 1.434 GelBank

35. Lab 1.435 Let’s Try it... http://www.gelscape.ualberta.ca