1. DNA Replication The process to create a second equivalent DNA molecule from one original

3. Steps in DNA Replication Process Helicase unzips the DNA Binding proteins keep single strand DNA “unzipped” DNA Polymerase build leading strand in 5’ to 3’ direction RNA primer on lagging strand begins and DNA Polymerase continues to build in discontinuous fragments DNA Ligase joins the Okazaki fragments on the lagging strand DNA Polymerases for elongation (delta and epsilon) proof read the DNA

4. PCR-Polymerase Chain Reaction The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.