1. POIWG #32 – Jul. 24-26, 2012 François NUYTTENS – Cécile THEVENOT Overview of the Inc.33-34 Experiments supported by CADMOS

2. 2 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville REVERSIBLE FIGURES: Perceived reversals of perspective Reversible Figures ENERGY: Astronaut’s energy requirements for long-term space flights NEUROSPAT: Prefrontal brain functions and spatial cognition MARES Commissioning Part 2: Operational test of MARES with a man in the loop BDC only: EDOS: Early Detection of Osteoporosis in Space (ground only) SPIN-GAZE: Centrifugation, static tilt and orthostatic tilt tests (ground only) CARTILAGE: Effect of microgravity on cartilage morphology and biology (ground only) “Specials”: DOSIS-3D: Dose Distribution inside ISS (support to MUSC) ICV: Integrated Cardiovascular (support to NASA-ICV for EPM CBPD) VESSEL IMAGING: Vascular Imaging (support to DAMEC) Increments 33-34 SUPPORTED ACTIVITES

3. 3 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville Ops LeadExperiments (activity prime) Stéphanie HerrENERGY, MARES, NEUROSPAT, REVERSIBLE FIGURES, Alain MailletEPM, DOSIS-3D, ENERGY, MARES, VESSEL IMAGING Maurice MarnatNEUROSPAT, REVERSIBLE FIGURES François NuyttensENERGY, MARES, NEUROSPAT, REVERSIBLE FIGURES Lourdes Oro MarotDOSIS-3D, MARES, ENERGY, REVERSIBLE FIGURES Hélène RugetMARES, ENERGY, EPM, NEUROSPAT, DOSIS-3D Cécile ThevenotENERGY, DOSIS-3D, EPM, VESSEL IMAGING Stéphanie François Hélène Cécile Maurice CADMOS OPS Team Lourdes Alain

4. 4 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville REVERSIBLE FIGURES Perceived reversals during visualization of perspective-reversible figures Science overview The objective of this experiment is to investigate whether the perception of ambiguous figures is affected by microgravity. The experiment will compare the percept reversals during the visualization of perspective-reversible figures in astronauts before, during and after long-term duration exposure to microgravity. Free floating required Main Hardware ESA MultiPurpose Payload Laptop Visor, Trackball, dedicated Hard disk, PCMCIA Card Subjects Inc 33-34: S. Williams, K. Ford and C. Hadfield (Prime) T. Marshburn (reserved) Second subject out of 6 Implementation Up to 6 sessions per subject, 4 prime + 2 reserve Crew time 65 min per session + 10 min after last session for data downlink Data Data stored on experiment hard disk and PCMCIA card downlink to ground via OCA Photo / Video Video desired for ops support and science requirements check

5. 5 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville REVERSIBLE FIGURES Perceived reversals during visualization of perspective-reversible figures Head mounted display (visor) Setup in Columbus starboard cone Hard Disk and Ultrabay Adapter Trackball

6. 6 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville

7. 7 NEUROSPAT Prefrontal brain functions and spatial cognition Science overview Subject in free floating using a tunnel (lighshield) to immerge into challenging scene. EEG, EOG, EMG and ECG signals are acquired via EPM-MEEMM. Main Hardware EPM, MEEMM, laptops, lightshield, gamepad, EEG cap, consumable (gel), chest belt Subjects Inc 33-34: C.Hadfield (Subject), T.Marshburn (Operator) 5th subject (meet minimum requirement) Implementation 2 sessions per subject (around FD15 and FD60) 3 days activities /session Crew time Day 1 : hardware pre installation Day 2 : Electronic hardware activation, EEG cap setup, experimental session, quick stowing Day 3 : data saving, stowing – data downlink Data Data stored on MEEMM and laptop Neurosciences Hard Disks Data downlink via EPM High Rate link Photo / Video 5 min video required, photo-modeling pictures of EEG cap

8. 8 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville NEUROSPAT Prefrontal brain functions and spatial cognition

9. 9 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville ENERGY Astronaut’s energy requirements for long-term space flights (support to DAMEC) Science overview Measure energy requirements before and during long-term space flight (>90 days), through doubly labeled water (DLW) intake to measure total energy expenditure (TEE) and body composition Main Hardware DAMEC: PFS (in HRF2) including MBS system, PFS Face Mask Kit, Energy kit CADMOS: EPM & EPM Laptop, Energy Armband Kit, Energy Food Kit, Energy Urine Collection Kit, Energy Sample Kit, Energy Armband Consumable Kit, Energy UltraBay Wallet, Water Sampling Kit, Energy Water Kit, Urine Containment Bag Kit Coordinated ops with NASA for PFS, Water Sampling done from US P/L PWD only Subjects Inc 33-34: A.Hoshide (subject) Number of inflight subjects performed: 1 out of 9 Implementation 1 session of 11 days after 3 month in space Crew time Under USOS resources. 5.83hrs for PFS + 4.83hrs for ENERGY stand-alone, per test subject. Control: 3hrs Data Samples downloaded at ambient temperature, data downlinked via EPM Photo / Video N/A

10. 10 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville ENERGY Astronaut’s energy requirements for long-term space flights (support to DAMEC) Armband Energy Sample Kit Energy Water Kit Energy Armband Consumable Kit Energy Water Dispenser x10 ENERGY Food Kit Energy Urine Collection Kit ENERGY

11. 11 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville MARES Commissioning Part 2 Operational test of MARES with a man in the loop Commissioning overview Since MARES ESA commissioning Part 2 is aiming at checking MARES operability for human use, it will mainly be based on the SARCOLAB protocol in order to include a “man-in-the-loop” test protocol. This will allow the run of a realistic scenario with respect to operational aspects. Main Hardware DAMEC: NASA Ultrasound 2 device, HRF Rack CADMOS: MARES Payload, PEMS-2 (Percutaneous Electrical Muscle Stimulator), an ESA EMG (Electromyographic) device, EPM Laptop, and EPM Rack Subjects Subject: C. Hadfield and Operator: T. Marshburn Implementation Over 3 days of operations Crew time Data Data are copied from MARES hard disk to EPM Laptop and then from EPM Laptop downlinked to ground Photo / Video Video is highly desired CREW Crew time day 1 Crew time day 2 Crew time day 3 Total crew time Crew 1375280130785 Crew 21409015245 Total Crew 1 and 25153701451030 Operational time605570175Unit: minutes

12. 12 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville MARES Commissioning Part 2 Operational test of MARES with a man in the loop PEMS electrodes EMG electrodes Ultrasound MARES ankle configuration EMG signals Torque signal during PEMS stimulation

13. 13 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville ExperimentsPre-flightPost-flight ENERGY Between L-150 and L-30 DEXA scan b/w L-365d and L0 Body mass before launch Body mass between R+0 and R+7d DEXA scan between R+0d and R+1y REVERSIBLE FIGURES L-120 ±30d, L-90 ±30d, L-60 ±30d R+1 ±1d, R+4 ±2d, R+8 ±2d NEUROSPAT L-90d, L-60d, L-30d.R+2, R+6, >R+15 (x2) CARTILAGE 1 session on 2 days (blood x 2 days, urine x 2 days, MRI one of the 2 days) ideally in the 14 days pre-flight, no fixed date necessary (it is understood that it will need to be done before the crew’s last travel to GCTC R+1(-0/+1): early blood and urine collection (fasting) on 1 day only, no MRI R+ 2 to 7 days: Complete session (blood x 2 days, urine x 2 days, MRI one of the 2 days) on 2 days R+ 4 to 6 weeks: Complete session EDOS L-60d, L-30d R+1d (BM), R+2d (XCT), R+15d (BM), R+90d(BM & XCT), R+180d (BM & XCT), R+360d ±2w SPIN-GAZE <L (x3) the same Wk R+1d, R+4d, R+9d: VVIS and Tilt test Baseline Data Collection Overview EAC JSC GCTC JSC

14. 14 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville ENERGY: Astronaut’s energy requirements for long-term space flights REVERSIBLE FIGURES: Perceived reversals of perspective Reversible Figures NEUROSPAT: Prefrontal brain functions and spatial cognition – TBD contingency subject SARCOLAB: Myotendinous and Neuromuscular adaptation to long term spaceflight BDC only: EDOS: Early Detection of Osteoporosis in Space (ground only) SPIN-GAZE: Centrifugation, static tilt and orthostatic tilt tests (ground only) CARTILAGE: Effect of microgravity on cartilage morphology and biology “Specials”: BP Reg: Blood Pressure Regulation (support to CSA) DOSIS-3D: Dose Distribution inside ISS (support to MUSC) ICV: Integrated Cardiovascular (support to NASA-ICV for EPM CBPD) VESSEL IMAGING: Vascular Imaging (support to DAMEC) SUPPORTED ACTIVITES Increments 35-36

15. 15 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville BACKUP SLIDES THANK YOU FOR YOUR ATTENTION

16. 16 CADMOS – POIWG Inc33-34 I July 24th-26th, 2012 I Huntsville BPBlood PressureHRFHuman Research Facility CDLCardiolabLACSLeg/Arm Cuffs System ECGElectrocardiogramMEEMMMulti ElectroEncephalogramme Mapping Module EEGElectroencephalogramSWSoftware EOGElectrooculogram EPMEuropean Physiology Modules Facility HDHard Disk ACRONYMS