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Lentiviral shRNA silencing of murine bone marrow cell CCR2 leads to persistent knockdown of CCR2 function in vivo by Ilze Bot, Jian Guo, Miranda Van Eck,

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Presentation on theme: "Lentiviral shRNA silencing of murine bone marrow cell CCR2 leads to persistent knockdown of CCR2 function in vivo by Ilze Bot, Jian Guo, Miranda Van Eck,"— Presentation transcript:

1 Lentiviral shRNA silencing of murine bone marrow cell CCR2 leads to persistent knockdown of CCR2 function in vivo by Ilze Bot, Jian Guo, Miranda Van Eck, Peter J. Van Santbrink, Pieter H. E. Groot, Reeni B. Hildebrand, Jurgen Seppen, Theo J. C. Van Berkel, and Erik A. L. Biessen Blood Volume 106(4):1147-1153 August 15, 2005 ©2005 by American Society of Hematology

2 Silencing of CCR2 expression by pSUPER-H1.shCCR2. Ilze Bot et al. Blood 2005;106:1147-1153 ©2005 by American Society of Hematology

3 Silencing of CCR2 protein by pSUPER-H1.shCCR2. Ilze Bot et al. Blood 2005;106:1147-1153 ©2005 by American Society of Hematology

4 Efficient transduction of bone marrow cells using GFP lentivirus. Ilze Bot et al. Blood 2005;106:1147-1153 ©2005 by American Society of Hematology

5 Lentiviral shCCR2 results in reduced macrophage influx due to reduced CCR2 expression. Ilze Bot et al. Blood 2005;106:1147-1153 ©2005 by American Society of Hematology

6 Absence of nonspecific effects on related chemokine receptors. Ilze Bot et al. Blood 2005;106:1147-1153 ©2005 by American Society of Hematology

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