1. Today House Keeping –Final report (rubric, due date) –Insert, (your) primer walking. 15min.ppt fungal sequences, clones, NGS,CARC Edit, assemble Parasite Physa 5 (PP5)28S

2. Final Reports Topics – Snail/parasite identification (RT-PCR) – Fungal metagenomics Format: manuscript for submission – Format: Research note, Journal of Parasitology

3. REPORTS in PDF format Due 15.45h, Wednesday December 9th Key for final report Correct format (order/spacing/legends/etc.) Correct methods Basic results Appropriate references Discussion (analysis) 30% 20% 15% 25% of final grade for 446L (100/400) 20% of final grade for 546L (100/500)

5. clone3seq.zip

6. PARASITES AND SNAIL BIOLOGY “identity, possibilities” phylogenetics “intentions” transcriptomics PCR rDNA/mito Bioanalyzer DNA-free, direct sequencing gel electrophoresis nanodrop spec Sequence ID (BLAST) editing Phylogenetics electrophoresis RT-PCR gel CTAB Trizol TA cloning, B/W screening M13 sequencing Primer design, walking Qiagen plasmid extraction Restriction digests DNA RNA GenBank submission + Sevilleta snails

7. 15min.ppt

8. fungal sequences, clones, NGS,CARC

9. Edit, assemble Parasite Physa 5 (PP5)28S