1. 1 Usage of REST-GCA ZANG Zhenxiang zangzx416@gmail.com

2. 2 Outline Set data directory Set data directory Set ROI Set ROI Mask selection Mask selection Set order & select algorithm Set order & select algorithm Set covariates (optional) Set covariates (optional) Set output directory Set output directory Z score transformation Z score transformation

3. 3 Set directory Click the right button to add a directory: Click the right button to add a directory: ← →

4. 4 Set directory Add sub-folders Add sub-folders for batch analysis: for batch analysis: ← →

5. 5 Set directory Remove the selected directory: Remove the selected directory: ← →

6. 6 Set directory Remove all the directories: Remove all the directories: ← →

7. 7 Set ROI Voxel-wise ROI-wise

8. 8 Voxel-wise →

9. 9 Voxel-wise → → Please see REST-sliceviewer for details. The text file must contain only one column. Each line represents the value of a time point. →

10. 10 Set ROI Voxel-wise ROI-wise

11. 11 ROI-wise (between two ROIs) Set the first ROI: Set the first ROI: →

12. 12 ROI-wise → → Please see REST-sliceviewer for details. The text file must contain only one column. Each line represents the value of a time point. →

13. 13 ROI-wise Three ROIs have been added

14. 14 Mask selection → → →

15. 15 User defined mask Select your Select your mask: mask: ←

16. 16 Order and algorithm Set order: Set order: Choose the algorithm: Choose the algorithm: or

17. Select the Coefficient Mode 17 → Bivariate coefficients is for bivariate cGCA computation. Multivariate coefficients is for multivariate cGCA computation. The two selection are based on coefficient-based ROI-wise GCA.

18. 18 ROI-wise for bivariate c-GCA ROI_sequence: save the sequence of pair ROI for bivariate GCA. Three pairs of computation have been performed: ROI1 v.s. ROI2; ROI1 v.s. ROI3 and ROI2 v.s. ROI3. Bivariate Results: Order was 1. Left column is the signed-path coefficients (ROI1 v.s. ROI 2,… see ROI_sequence). Right column is the auto regression coefficients. → outputs

19. 19 ROI-wise for Multivariate c-GCA Order1 results: Order2 results:

20. 20 Add covariates Covariates are optional Covariates are optional ↓ → The covariates text file may contain several columns, each respresenting a covariate.

21. 21 Set output directory → ←

22. Z score transformation 22 The result maps will be renamed with suffix letter ‘Z’

23. 23 Click ‘Compute’ to start

24. 24 Thanks for using REST and REST-GCA! Thanks for using REST and REST-GCA!