1. Comparison of Mouse Data to Human Datasets 3/1/16

2. Mouse Dataset 452 genes DE in tumor vs. liver (p<0.01)

3. Heat Map Tumor v. Liver

4. Mouse Eigengene (452 DE genes)

5. Mouse v. Human GSE63898: 228 tumor samples, 168 cirrhosis samples (not matched) – 5514 de genes in tumor v. cirrhosis – 198 genes de in mouse and human datasets (p<0.01) GSE62232: 81 tumor samples, 10 normal liver (not matched) – 6148 de genes in tumor v. liver (human) – 158 genes de in mouse and human data sets (p<0.01) 105 genes in common between the two sets

6. Pathways for Genes in DE in mouse and both human data sets

7. GSE63898 217 HCC 179 Cirrhosis

8. GSE63898

12. Module 15 80 genes

13. Module 15 KEGG Pathways: Polyphyrin and chlorophyll metabolism Base excision repair SNARE interactions in vesicular transport GO Terms: Cellular response to zinc ion Cellular response to cadmium ion Cellular response to metal ion

14. Module 18 68 genes

15. Module 18 KEGG Pathways: Cell Adhesion Molecules Gastric acid secretion Bile secretion ECM-receptor interaction Prostate Cancer Wnt signaling

16. Module 15 Eigengenes for Mouse

17. Module 15 Projected on Mouse

18. Module 18 Eigengenes for Mouse

19. Module 18 Projected on Mouse

20. Mouse Samples Clustered Based on Eigengene Values for Each Module

21. Eigengene Modules Clustered Based on Mouse Samples

22. Module 5

23. 438 genes Module 5

24. KEGG Pathways: Fatty acid degradation Valine, leucine, isoleucine degradation PPAR signaling pathways Propanoate metabolism Cyp P450 Metabolsim of xenobiotics Beta-Alanine metabolism Trypotophan metabolism Alanine, aspartate, and glutamate metabolism Butanoate metabolism Glyoxylate and dicarboxylate metabolism Lysine degradation Peroxisome Glycine, serine, threonine metabolism Arginine, proline metabolism Tyrosine metabolism Retinol metabolism Etc.

25. GSE62232 81 HCC Samples 10 Normal Liver

26. Modules Samples

28. Module 5

29. Module 5 Eigengenes for Mouse

30. Module 5 Projected on Mouse 652 Genes

31. Module 5 KEGG Pathways: mRNA surveillance pathway Glycosylphosphatidylinositol (GPI)-anchor biosynthesis Protein processing in ER N-glycan biosynthesis Lysine degradation RNA transport Pyrimidine metabolism

32. Module 14 Eigengenes for Mouse

33. Mouse Samples Clustered Based on Eigengene Values for Each Module

34. 154 genes Module 14 Projected on Mouse

35. Module 14 KEGG Pathways: Ameobiasis Focal adhesion Pathways in cancer ECM-receptor interaction Small cell lung cancer Gap junction Melanoma MAPK signaling pathways Protein digestion and absorption Leukocyte transendothelial migration Vascular smooth muscle contraction B cell receptor signaling pathway Cell adhesion molecules Regulation of actin cytoskeleton Hematopoietic cell lineage GO Terms: Cardiovascular system development Blood vessel development Angiogenesis Tissue development Cell migration Cell motility

36. Mouse Eigengene Projected on Human Datasets GSE63898 GSE62232