1. Plate goniometers

5. 1 μm crystal≈ 1 μm water ≈ 1 μm plastic ≈ 0.1 μm glass ≈ 1000 μm air scattering/absorptoin “rules”: Optimum: support thickness = crystal thickness

6. NatX-ray CrystalQuick™ X 250 μm ~50 μm tape Fluidigm Topaz® DC 200 μm PDMS MiTeGen in-situ-1 100 μm ~50 μm tape in-situ diffraction trays CrystalDirect ~50 μm tape 25 μm Microlytic Crystal Former™ 1000 μm

7. Example Data lysozyme 50 μm beam 37 Gy/s (0.775 Å) 30s exposures at 20C 90° of data, 97% complete I/  = 1.5 at 1.9 Å R merge 18% (overall) 5% (low) ΔB same as 20 min at 100K B factor -2 -4 -6 scale vs batch 210210

8. Data Collection Results Protein Space group rot Range (°)Temp. Mosaic (°) Max res (Å) R sym (%) Complete (%) Thaumatin P4 1 212180100K0.061.310.0597.8 P4 1 21240100K0.081.380.03792.4 Ferritin F4 3 223RT0.0720.1797.7 F4 3 245100K0.420.17499.9 F4 3 260100K0.420.20999.4 Glucose Isomerase I22240100K0.151.480.09882.3 I22240100K0.311.30.04961.8 Insulin R360100K0.151.80.1186.9 I2 1 390100K0.12.20.2898.3 I2 1 3100100K0.12.20.396

9. Zero-parallax microscope pinhole mirror Styrofoam™ backlight backstop microscope

10. Zero-parallax microscope pinhole mirror Styrofoam™ backlight backstop microscope

11. Zero-parallax microscope pinhole mirror microscope

12. Zero-parallax microscope pinhole mirror microscope

13. Zero-parallax microscope pinhole mirror microscope

14. Crowfoot Cell

16. J. D. Bernal and D. Crowfoot, Nature (London) 133, 794 (1934).