1. Sources of Bacterial Species with Antibiotic Activity: Soil & Rotten Wood

2. Collect rotten wood aseptically

3. Culture bacteria from rotten wood

4. Streak bacterium for Isolation

5. Test Isolates for inhibition of pathogens

6. Gram stain isolate

7. Extract DNA from Isolates

8. PCR 16s rRNA gene

9. Run Gel to test for ample DNA

10. Send PCR product for sequencing >S1104113.NWC-2-CP1 NNNNNNNNNNNNNNNGCGGNTGGNTCCNAAAAGGNTACCCCACCGACTTCGGGTGTTACAAACTCTC GTGGTGTGNNNNNNNNTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACT AGCGATTCCAGCTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGAACGGTTTTATGAGATTAGCT CCACCTCGCGGTCTTGCAGCTCTTTGTACCGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCAT GATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTTAATGA TGGCAACTAAGATCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGAC AACCATGCACCACCTGTCACTCTGCTCCCGAAGGAGAAGCCCTATCTCTAGGGTTTTCAGAGGATGTCAAG ACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATT CCTTTGAGTTTCAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAACTTCAGCACTAAAGG GCGGAAACCCTCTAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTC CCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGAAAGTCGCCTTCGCCACTGGTGTTCCTCCATATCT CTACGCATTTCACCGCTACACATGGAANTCCACTTTCCTCTTCTGCACTCAAGTCTCCCAGTTTCCAATGACC CTCCACGGTTGAGCCGTGGGCTTTCACATCAGACTTAAGAAACCACCTGCGCGCGCTTTACGCCCAATAAT TCCNNANNACGCTTGCNANCTACGTATTACNGCGNCTGCTGGCNCGTAGTTAGCNNNNGNTTTCTGGNT AGGTACCGNCANNNNCCAGNTNNNTNANNAGNNNTNGTNNTNCNNANNNANNGANTTNACNACCC NNANNCTNNNTCACTCNNNNNNNGTTGCN

11. Use sequence for BLAST search

12. Search journals

13. You’ve found a new antibiotic!

14. Was the difference significant? Tundra SoilRotting Wood Total Tested460468 Isolates with Activity 54 81

15. Two variable Z test for population proportions

16. p value = 0.0087

17. Cupressaceae vs alternative tree families

18. Future Efforts

19. Wyoming INBRE Northwest College Mentors: Dr. A. Childs, Dr. S. Harbron, and Dr. E. Kimble. Sue Norris (Statistics) Fellow Students