1. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Fig. 7.6 The Replication Fork Slide number 1 5' 3' 5' Synthesis of the leading strand proceeds continuously. Synthesis of the lagging strand is discontinuous; synthesis is reinitiated periodically, generating a series of fragments that are later joined. 1. Primase synthesizes an RNA primer 2. DNA polymerase adds nucleotides onto the 3' end of the fragment 3. DNA polymerase replaces the RNA primer with deoxynucleotides 3' 5' Helicase separates the double-stranded molecule RNA primer 4. DNA ligase seals the gaps between adjacent fragments

2. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Fig. 7.6 The Replication Fork Slide number 2 5' 3' Helicase separates the double-stranded molecule

3. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Fig. 7.6 The Replication Fork Slide number 3 5' 3' Synthesis of the leading strand proceeds continuously. 3' 5' Helicase separates the double-stranded molecule

4. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Fig. 7.6 The Replication Fork Slide number 4 5' 3' Synthesis of the leading strand proceeds continuously. Synthesis of the lagging strand is discontinuous; synthesis is reinitiated periodically, generating a series of fragments that are later joined. 1. Primase synthesizes an RNA primer 3' 5' Helicase separates the double-stranded molecule RNA primer

5. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Fig. 7.6 The Replication Fork Slide number 5 5' 3' Synthesis of the leading strand proceeds continuously. Synthesis of the lagging strand is discontinuous; synthesis is reinitiated periodically, generating a series of fragments that are later joined. 1. Primase synthesizes an RNA primer 3' 5' Helicase separates the double-stranded molecule 2. DNA polymerase adds nucleotides onto the 3' end of the fragment RNA primer

6. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Fig. 7.6 The Replication Fork Slide number 6 5' 3' 5' Synthesis of the leading strand proceeds continuously. Synthesis of the lagging strand is discontinuous; synthesis is reinitiated periodically, generating a series of fragments that are later joined. 1. Primase synthesizes an RNA primer 3. DNA polymerase replaces the RNA primer with deoxynucleotides Helicase separates the double-stranded molecule 2. DNA polymerase adds nucleotides onto the 3' end of the fragment RNA primer

7. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Fig. 7.6 The Replication Fork Slide number 7 5' 3' 5' Synthesis of the leading strand proceeds continuously. Synthesis of the lagging strand is discontinuous; synthesis is reinitiated periodically, generating a series of fragments that are later joined. 1. Primase synthesizes an RNA primer 3. DNA polymerase replaces the RNA primer with deoxynucleotides 3' 5' Helicase separates the double-stranded molecule 2. DNA polymerase adds nucleotides onto the 3' end of the fragment RNA primer 4. DNA ligase seals the gaps between adjacent fragments