1. LAB 5 Cellular Respiration http://www.phschool.com/science/biology_place/labbench/lab5/process.html

2. C H O + 6O → 6 H O + 6 CO KOH turns CO gas into a solid So changes in volume will be due to ? 2 http://www.phschool.com/science/biology_place/labbench/lab5/respwork.html 6 12 6 2 2 2

3. CONTROL If you want to control variables the contents of the vials must start the SAME How can you measure the volume of an irregularly shaped object, like peas?

4. http://www.phschool.com/science/biology_place/labbench/lab5/assemble.html Make another set 1, 2, 3 will go in room temp; 4, 5, 6 will go in 10° water

5. http://www.phschool.com/science/biology_place/labbench/lab5/measure.html Place respirometers in water bath with tips of pipettes resting on a masking tape sling above the water level. Wait 10 MINUTES for temperature to equilibrate. Add a drop of FOOD COLOR into the tip of each pipette

6. http://www.phschool.com/science/biology_place/labbench/lab5/measure.html Lower the pipettes into water Quickly align the tubes so you can read the numbers Wait 3 MINUTES Record level of water in each pipette and start timer Take readings from each respirometer at 5 minute intervals for 20 minutes REPEAT with 2 nd set of respirometers in ice water

8. 0.47 0.45 0.02 0.650.51 1.020.35 0.37 0.53 0.02 0.470.021.62 0.00 0.970.950.600.09 0.07 0.47 0.022.17 1.52 1.500.630.120.11 0.47 0.02 2.882.23 2.21 0.70 0.190.18 25° 10°

9. http://www.phschool.com/science/biology_place/labbench/lab5/quiz.html

10. HOW DOES TEMPERATURE AFFECT THE RESPIRATION RATE IN AN ORGANISM? http://www.uselessgraphics.com/rats.htm http://www.lbreptileexperience.com/adventures.html Cold blooded (Ectothermic) Warm blooded (Endothermic)