Presentation is loading. Please wait.

Presentation is loading. Please wait.

Hybrid systems with nanocellulose: Magnetic responsive nanocellulose for separation technology applications - Protein separation Puu-0.4210 – Nanotechnology.

Published byBasil Shepherd Modified over 8 years ago

Similar presentations

Presentation on theme: "Hybrid systems with nanocellulose: Magnetic responsive nanocellulose for separation technology applications - Protein separation Puu-0.4210 – Nanotechnology."— Presentation transcript:

1 Hybrid systems with nanocellulose: Magnetic responsive nanocellulose for separation technology applications - Protein separation Puu-0.4210 – Nanotechnology in Forest Biomaterials Nanna Koivula Advisor: Ilari Filpponen

2 Introduction Magnetic nanoparticles used in protein separation -> medical applications, affinity filtration etc. (Moreno-Guzmán et al. 2010)

3 Papain recovery Papain recovery- CNC/Fe 3 O 4 NPs/AuNPs Magnetic core: magnetite nanoparticles (Fe 3 O 4 NPs) Metal or metal oxide shell: Au nanoparticles (AuNPs) (Mahmoud et al. 2013)

4 Approach Materials TEMPO-ox. cellulose nanocrystals (CNC) Magnetic nanoparticle (Fe 3 O 4 ) Methods Covalent attachment of magnetic nanoparticles to the TEMPO-CNC CNC modification to provide specific affinity towards target protein (addition of Cu2+ forms a ligand which is specific for lysozyme binding; (Zhu, J.; Sun, G (2014)) Fluorescence labelling of protein (for demo purposes) Application Protein isolated from sample solution under magnetic field

5 References Mahmoud, K. Lam, E. Hrapovic, S. Luong, J. 2013. Preparation of Well-Dispersed Gold/Magnetite Nanoparticles Embedded on Cellulose Nanocrystals for Efficient Immobilization of Papain Enzyme. ACS applied materials & interfaces.5:11. pg. 4978–4985 Moreno-Guzman, M. Eguılaz, M et al. 2010. Disposable immunosensor for cortisol using functionalized magnetic particles. Analyst. 135, pg. 1926-1933 Nacev, A. Beni, C. Bruno, O. Shapiro, B. 2010. Magnetic nanoparticle transport within flowing blood and into surrounding tissue. 5:11. pg. 1459-1466. Nypelo, T. Rodriguez-Abreu, C. Rivas, J. Dickey, M, Rojas. O. 2014. Magneto-responsive hybrid materials based on cellulose nanocrystals. Cellulose. 21. pg. 2557–2566 Peng, J. Liu, Q. Xu, Z. Masliyah, J. 2012. Synthesis of Interfacially Active and Magnetically Responsive Nanoparticles for Multiphase Separation Applications. Advanced functional materials. 22:8. pg. 1732-1740. Zhu, J.; Sun, G. 2014. Facile Fabrication of Hydrophilic Nanofibrous Membranes with an Immobilized Metal–chelate Affinity Complex for Selective Protein Separation. ACS applied materials & interfaces, 6, pg. 925-932.

Download ppt "Hybrid systems with nanocellulose: Magnetic responsive nanocellulose for separation technology applications - Protein separation Puu-0.4210 – Nanotechnology."

Similar presentations

Mechanical and Industrial Engineering University of Massachusetts Amherst, MA, USA Nanomedicine Jonathan P. Rothstein.

Mechanical and Industrial Engineering University of Massachusetts Amherst, MA, USA Nanomedicine Jonathan P. Rothstein.
Mechanical and Industrial Engineering University of Massachusetts Amherst, MA, USA Nanomedicine Jonathan P. Rothstein.

Mechanical and Industrial Engineering University of Massachusetts Amherst, MA, USA Nanomedicine Jonathan P. Rothstein.
Molecular Biomimetics Polypeptides to Inorganic structures.

Molecular Biomimetics Polypeptides to Inorganic structures.
Overview energy is required for all cellular work most organisms produce ATP by using energy stored in the bonds of organic molecules such as carbohydrates.

Overview energy is required for all cellular work most organisms produce ATP by using energy stored in the bonds of organic molecules such as carbohydrates.
Application of Silicon Nanowire in Biosensor Student: HongPhan ID: 9735582 Professor: Cheng-Hsien Liu.

Application of Silicon Nanowire in Biosensor Student: HongPhan ID: Professor: Cheng-Hsien Liu.
The study of cysteine molecule coated magnetic Fe 3 O 4 nanoparticles via sonochemical method for bio-applications Kevin J. Schilling, Joo Seob Lee, and.

The study of cysteine molecule coated magnetic Fe 3 O 4 nanoparticles via sonochemical method for bio-applications Kevin J. Schilling, Joo Seob Lee, and.
NSF-ITR Meeting 10-05 Miniaturized Sensors Air molecules aerosols Water molecules cells, viruses.

NSF-ITR Meeting Miniaturized Sensors Air molecules aerosols Water molecules cells, viruses.
Chem 5395 J. Rusling Fall 2009 Introductory Materials “interfacial properties differ from bulk properties” Phase 1Phase 2.

Chem 5395 J. Rusling Fall 2009 Introductory Materials “interfacial properties differ from bulk properties” Phase 1Phase 2.
Click to edit Master subtitle style CSIRO IT Progress towards the measurement of a small spin system using a nanoSQUID January 2006 S.K.H. Lam, W. Yang,

Click to edit Master subtitle style CSIRO IT Progress towards the measurement of a small spin system using a nanoSQUID January 2006 S.K.H. Lam, W. Yang,
Metal Chelate Affinity Chromatography Wenbo Dong Yagmur Yagdiran.

Metal Chelate Affinity Chromatography Wenbo Dong Yagmur Yagdiran.
Nucleation of gold nanoparticles on graphene from Au 144 molecular precursors Andrei Venter 1, Mahdi Hesari 2, M. Shafiq Ahmed ­1, Reg Bauld 1, Mark S.

Nucleation of gold nanoparticles on graphene from Au 144 molecular precursors Andrei Venter 1, Mahdi Hesari 2, M. Shafiq Ahmed ­1, Reg Bauld 1, Mark S.
Nanocellulose in conductive materials Lauri Matikainen.

Nanocellulose in conductive materials Lauri Matikainen.
Magnetic applications of nanocellulose

Magnetic applications of nanocellulose
Événement Parlons Affaires Photonics North 2014, Montréal, 29 mai.

Événement Parlons Affaires Photonics North 2014, Montréal, 29 mai.
Nature Nanotechnology, January 2011 Part I: Understanding Tissue.

Nature Nanotechnology, January 2011 Part I: Understanding Tissue.
Magnetic Core/Shell Nanocomposites Mohamed Darwish Institute of Nanomaterials, Advanced Technology and Innovation Technical University of Liberec 23/4/2013.

Magnetic Core/Shell Nanocomposites Mohamed Darwish Institute of Nanomaterials, Advanced Technology and Innovation Technical University of Liberec 23/4/2013.
LUMIER-Assays Dr. Manfred Koegl Protein Interaction Unit

LUMIER-Assays Dr. Manfred Koegl Protein Interaction Unit
Objective DNA amplification (eg. PCR)    advances in forensic, clinical applications Comparable protein amplification tools (?)  aid medical diagnostics,

Objective DNA amplification (eg. PCR)    advances in forensic, clinical applications Comparable protein amplification tools (?)  aid medical diagnostics,
Projects in the research area Hybrid bionanosystems based on self-assembled structures, quantum dots, plasmonic and magnetic nanoparticles Strategic Line.

Projects in the research area Hybrid bionanosystems based on self-assembled structures, quantum dots, plasmonic and magnetic nanoparticles Strategic Line.
Cell Membranes Recall that the smallest unit of life is the cell. All cells are surrounded by a plasma membrane that is vital in various aspects of the.

Cell Membranes Recall that the smallest unit of life is the cell. All cells are surrounded by a plasma membrane that is vital in various aspects of the.

Similar presentations

Ads by Google