1. Optical System Review

2. Lens concept selections Solving the optical parameters (Magnification) (Focal length), Where, S’ is the distance from the lens to image plane S is the distance from the grid to the lens M is the magnification. Box Length = 250 mm Box Height = 200 mm.

3. Lens Types and Specifications

4. High Resolution Large Format Lens (Cost estimate: $500 - $1500)

5. Computar C mount (Cost estimate: $50 - $100)

6. OSLO Simulation

7. Software System Capabilities Consist of three separate programs Kodak Molecular Imaging Software Optigrid Motor Control GUI MATLAB –Scripts will be developed as necessary for calibration and depth resolved discrimination Optigrid Calibration –A system specific calibration process will be developed –Process will rely on manual coordination of three separate programs Implement necessary algorithms to achieve depth discrimination, as described by Tromberg et al. in US Publication 2006/0184043

9. Objective: To mathematically investigate and invoke the necessary algorithms to achieve the depth discrimination, as described by Tromberg et al. in US Publication 2006/0184043 Bevilacqua, F., Cuccia, D., Durkin, A., Tromberg, B., “Quantitative analysis and imaging of subsurface heterogeneities using spatially structured illumination,” 2002.

10. Phantom Design Objective: To develop a stable, long-term phantom whose optical properties can be predetermined and reproduced, at selected wavelengths, of human biological tissues of the hand, over a spectral range between 400 and 1,000 nm. Purpose: –Simulate light distributions with a geometry of physical tissue. –Initially testing system designs –Calibration of optical devices. –Create reference measurements of reflective fluorescence. How: –Understand of the key physical and biochemical characteristics of tissue that influence its interaction with light. –Match the absorption coefficient μ a, scattering coefficient μ s, and the anisotropic coefficient g(λ)

11. Design of Phantom 3 components: –Casting material Polyurethane –Absorbing material phthalocyanin dye (Epolight 6084) –Scattering agent Aluminum Oxide (alumina; Al 2 O 3 )

13. Total attenuation coefficient, as a function of concentration, of 0.954-mm-diameter micro-spheres.