1. Introduction to Muscle Tissue

2. Skeletal Muscle - red fibres - white fibres - intermediate fibres Cardiac Muscle - cardiomyocytes - Purkinje fibres Smooth Muscle Myoepithelial Cells Other cells

3. Introduction to Muscle Tissue Skeletal Muscle - red fibres - white fibres - intermediate fibres Cardiac Muscle - cardiomyocytes - Purkinje fibres Smooth Muscle Myoepithelial Cells Other cells (including myofibroblasts) -overall shape -HAZI structure -triads -connective tissue elements -overall shape -intercalated disk -diads -communication and coordination -overall design and function Hypertrophy vs. Hyperplasia

4. Skeletal Muscle - red fibres - white fibres - intermediate fibres

5. Skeletal Muscle - red fibres - white fibres - intermediate fibres

6. Skeletal Muscle - red fibres - white fibres - intermediate fibres It’s HAZI to me!

7. Skeletal Muscle

12. Skeletal Muscle Connective Tissue Layers endomysium perimysium epimysium (not shown) Muscle spindle apparatus

13. Skeletal Muscle

14. Cardiac Muscle – cross section

15. Cardiac Muscle – longitudinal section

16. Cardiac Muscle – tangential section

17. Cardiac Muscle – longitundinal, higher power section

18. Cardiac Muscle – longitundinal, higher power view

19. Cardiac Muscle – longitundinal, higher power electron microscopic view

20. Cardiac Muscle – Purkinje fibres

21. Cardiac Muscle – Purkinje fibres and A-V node

22. Smooth Muscle – LM and TEM

23. Smooth Muscle – various angles of presentation

25. Smooth Muscle – nuclear wiggles

26. Myoepithelial Cells – rapid glandular release

27. Lab #2 – Muscle Tissue

28. Objectives -Learn how slides are made in more detail -Introduction to histological stains -Skeletal muscle – and white muscle disease -Cardiac muscle – and sarcocysts -Smooth Muscle – and myometrial hyperplasia

29. Lab #2 – Muscle Tissue how slides are made 1.Tissue preservation 2.Clearing and dehydration 3.Embedding in paraffin or plastic 4.Sectioning – expanding on a warm water bath 5.Mounting on a glass slide 6.De-waxing 7.Staining 8.Coverslipping

32. How are histology slides made?

34. fixation processing slicing and mounting de-waxing, staining hematoxylin & eosin osmium Masson’s Trichrome cover slips

35. How are histology slides made? fixation processing slicing and mounting de-waxing, staining hematoxylin & eosin osmium Masson’s Trichrome cover slips

36. How are histology slides made? fixation processing slicing and mounting de-waxing, staining hematoxylin & eosin osmium Masson’s Trichrome cover slips

37. How are histology slides made? fixation processing slicing and mounting de-waxing, staining hematoxylin & eosin osmium Masson’s Trichrome cover slips

38. How are histology slides made? fixation processing slicing and mounting de-waxing, staining hematoxylin & eosin osmium Masson’s Trichrome cover slips

39. How are histology slides made? fixation processing slicing and mounting de-waxing, staining hematoxylin & eosin osmium Masson’s Trichrome cover slips

40. How are histology slides made? fixation processing slicing and mounting de-waxing, staining hematoxylin & eosin osmium Masson’s Trichrome cover slips

41. How are histology slides made? fixation processing slicing and mounting de-waxing, staining hematoxylin & eosin osmium Masson’s Trichrome cover slips

42. Lab #2 – Muscle Tissue Histological Staining - hematoxylin & eosin: general cellular detail - Masson’s trichrome: collagen - Wright’s blood stain: granulocytes - osmium tetroxide: lipid - silver nitrate: reticular fibres - periodic acid Schiff: glycogen and other carbohydrates Fixatives for Histology - formalin - other aldehydes - osmium tetroxide - air drying - alcohols - freeze fixation

43. Lab #2 – Muscle Tissue Histological Staining - hematoxylin & eosin: general cellular detail - Masson’s trichrome: collagen - Wright’s blood stain: granulocytes - osmium tetroxide: lipid - silver nitrate: reticular fibres - periodic acid Schiff: glycogen and other carbohydrates Fixatives for Histology - formalin - other aldehydes - osmium tetroxide - air drying - alcohols - freeze fixation

44. Lab #2 – Muscle Tissue Histological Staining - hematoxylin & eosin: general cellular detail - Masson’s trichrome: collagen - Wright’s blood stain: granulocytes - osmium tetroxide: lipid - silver nitrate: reticular fibres - periodic acid Schiff: glycogen and other carbohydrates Fixatives for Histology - formalin - other aldehydes - osmium tetroxide - air drying - alcohols - freeze fixation