1. Date of download: 7/7/2016 Copyright © The American College of Cardiology. All rights reserved. From: Relaxin-2 and Soluble Flt1 Levels in Peripartum Cardiomyopathy: Results of the Multicenter IPAC Study JCHF. 2016;4(5):380-388. doi:10.1016/j.jchf.2016.01.004 Biomarker Levels by Days Post-Partum (Quartiles) (A) Relaxin-2 levels (pg/ml) were significantly higher in women presenting in the first quartile (0 to 11 days) compared with subjects presenting in the latter 3 quartiles (12 to 95 days) (p = 0.0043). (B) Prolactin levels (ng/ml) were significantly higher in women presenting in the first quartile compared with subjects presenting in the latter 3 quartiles (p < 0.00001). (C) Soluble fms-like tyrosine kinase 1 (soluble Flt1) levels (pg/ml) were significantly higher in women presenting in the first quartile compared with subjects presenting in the latter 3 quartiles (p < 0.003). (D) Vascular endothelial growth factor (VEGF) levels (pg/ml) were not significantly different by days post-partum (p = 0.73). Boxplots: box represents the interquartile range from first to third quartiles. Median represented by a thick black line across the interquartile range. Error bars represent 1.5 times the interquartile range. Figure Legend:

2. Date of download: 7/7/2016 Copyright © The American College of Cardiology. All rights reserved. From: Relaxin-2 and Soluble Flt1 Levels in Peripartum Cardiomyopathy: Results of the Multicenter IPAC Study JCHF. 2016;4(5):380-388. doi:10.1016/j.jchf.2016.01.004 LVEF Over Time by Tertiles of Relaxin-2 Level at Entry (A) Entire cohort (n = 98). White bars = lowest tertile; gray bars = intermediate tertile; red bars = highest tertile. Early myocardial recovery (left ventricular ejection fraction [LVEF] at 2 months) best in the highest relaxin tertile (p = 0.01). (B) Subjects enrolled in the first quartile of days post-partum (0 to 11 days, n = 25). White bars = lowest tertile; gray bars = intermediate tertile; red bars = highest tertile. Early myocardial recovery (LVEF at 2 months) again greatest in the highest relaxin tertile (p = 0.02). Figure Legend:

3. Date of download: 7/7/2016 Copyright © The American College of Cardiology. All rights reserved. From: Relaxin-2 and Soluble Flt1 Levels in Peripartum Cardiomyopathy: Results of the Multicenter IPAC Study JCHF. 2016;4(5):380-388. doi:10.1016/j.jchf.2016.01.004 Soluble Flt1 Levels by NYHA Functional Class Boxplots with red box representing the interquartile range from the first to the third quartile. Median represented by a thick black line across the interquartile range. Error bars represent 1.5 times the interquartile range. Higher progressive soluble Flt1 levels with increasing NYHA functional class (p = 0.01). Flt1 = fms-like tyrosine kinase 1; NYHA = New York Heart Association. Figure Legend:

4. Date of download: 7/7/2016 Copyright © The American College of Cardiology. All rights reserved. From: Relaxin-2 and Soluble Flt1 Levels in Peripartum Cardiomyopathy: Results of the Multicenter IPAC Study JCHF. 2016;4(5):380-388. doi:10.1016/j.jchf.2016.01.004 Event-Free Survival by sFlt1 Tertiles Event-free survival was defined as free of death, cardiac transplantation, or left ventricular assist device implantation. Kaplan-Meier log-rank analysis comparing subjects in the highest tertile of soluble Flt1 levels with subjects in the lower 2 tertiles. Poorer event- free survival was evident for subjects with higher soluble Flt1 level (p = 0.004). Flt1 = fms-like tyrosine kinase 1. Figure Legend: