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Albumin MIP Prepared in Aqueous Solution System Speaker : Thong-Yueh Lin 報告者 : 林 聰 樂
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Outline Introduction Experimental Results and discussion Conclusions
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Introduction
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Property of Albumin Half-life: 17 to 23 day Molecular weight: 68,000 Isoelectric point: 4.8 Concentration of human serum: 3.0 to 5.0 g/dl
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Experimental
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Materials Monomer 3-dimethylaminopropyl metacrlamide:DMAPMA acrylamide:AMAllylamine:AA Methacrylic acid:MAA Hydroxy ethyl methacrylate:HEMA
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Crosslinking agent Tetraethylene glycol dimethacrylate:TEGDMA Polyethylene glycol dimethacrylate:PEGDMA Materials
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Initiators Ammonium persulfate:APS Cumene hydroperoxide:CHP Tetramethyl ethylenediamine:TMEDA Materials
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Fabrication Sequence of MIP Preparation of MIPcrush of MIP Screening of MIP Washing with 20 wt.% methanol and drying of MIP
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Results and discussion
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HPLC analysis of protein mixture
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Typical calibration curve of Cytochrome C by using HPLC
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Typical calibration curve of Albumin by using HPLC
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Typical calibration curve of Lysozyme by using HPLC
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Typical calibration curve of Myoglobin by using HPLC
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Selectivity mass ratio to adsorbent of M.I.P Sample name: DMAPMA-TEGDMA Albumin 4.5% Water 10.4% DMAPMA 5.0% TEGDMA 80% APS,TMEDA Selectivity: 1:1.9:14.2:1. 4
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Selectivity mass ratio to adsorbent of M.I.P Sample name: MAA-TEGDMA Albumin 3.1% Water 7.3% MAA 2.7% TEGDMA 86.8% APS,TMEDA Selectivity: 52.2:160.1: 1:77.5
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Selectivity mass ratio to adsorbent of M.I.P Sample name: AM-TEGDMA 1 Albumin 2.4% Water 9.2% AM 2.4% TEGDMA 86% APS,TMEDA Selectivity: 1:4.1:6.5:2.5
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Selectivity mass ratio to adsorbent of M.I.P Sample name: AM-TEGDMA 2 Albumin 2.4% Water 11.2% AM 2.3% TEGDMA 84% CHP,TMEDA Selectivity 4.4:25.8:8.9:1
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Selectivity mass ratio to adsorbent of M.I.P Sample name: HEMA-PEGDMA Albumin 4.5% Water 10.5% HEMA 5.1% PEGDMA 80% APS,TMEDA Selectivity 1: 4.3: 1: 1.8
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Selectivity mass ratio to adsorbent of M.I.P Sample name: DMAPMA-PEGDMA Albumin 5.4% Water 12.6% DMAPMA 6.0% PEGDMA 76% APS,TMEDA Selectivity 3.1:6.4:5.4:1
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Mass ratio of Adsorbent for different composition MIP Sample name Mass ratio of adsorbent (Cytochrome C: Lysozyme: Albumin: Myoglobin) DMAPMA-TEGDMA 1 : 1.9 : 14.2 : 1.4 MAA-TEGDMA 52.2 : 160.3 : 1 : 77.5 AM-TEGDMA 1 : 4.1 : 6.5 : 2.5 AM-TEGDMA 4.4 : 25.8 : 8.9 : 1 HEMA-PEGDMA 1 : 4.3 : 1 : 1.8 DMAPMA-PEGDMA 3.1 : 6.4 : 5.4 : 1
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Conclusions 1.The DMAPMA-TEGDMA MIP can obtain high selectivity for albumin selectivity for albumin 2.The mass ratio of DMAPMA-TEGDMA MIP for adsorbent = Albumin : Lysozyme : Myoglobin : Cytochrome C = Albumin : Lysozyme : Myoglobin : Cytochrome C = 14.2 : 1.9 : 1.4 : 1 = 14.2 : 1.9 : 1.4 : 1
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Thank you for your attention
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